Development of Anti-atypical Aeromonas salmonicida Monoclonal Antibodies for Diagnosis of "New Ulcer Disease" in Koi Carp
Development of Anti-atypical Aeromonas salmonicida Monoclonal Antibodies for Diagnosis of "New Ulcer Disease" in Koi Carp
复制标题
DOI：
10.3147/jsfp.53.36
复制
发表时间：
2018-03-01
期刊：
影响因子：
0.6
通讯作者：
Narita, Hiroshi
中科院分区：
文献类型：
--
作者：
Somamoto, Tomonori;Maruyama, Shinpei;Narita, Hiroshi
为了建立锦鲤“新溃疡病”的准确诊断方法，我们制备了4株抗锦鲤非典型杀鲑气单胞菌的单克隆抗体。这些单克隆抗体与非典型A.从日本比目鱼中分离出杀鲑素和其他病原菌。通过在考马斯亮蓝琼脂培养基上选择蓝色菌落，从人工感染的锦鲤中进行再分离，并对部分菌落进行免疫荧光染色。这些结果表明，单克隆抗体可以区分非典型A。杀鲑素从锦鲤从居民气单胞菌。
To develop an accurate diagnosis of "new ulcer disease" in koi carp, we produced four monoclonal antibodies (mAbs) against a strain of atypical Aeromonas salmonicida isolated from koi carp. These mAbs did not cross-react with an isolate of atypical A. salmonicida from Japanese flounder and other pathogenic bacteria. Re-isolation from artificially infected koi carp was achieved by selecting the blue colonies on agar medium containing Coomassie brilliant blue, and some of the colonies were detected by immunofluorescent staining using the mAbs. These results suggested that the mAbs can distinguish atypical A. salmonicida from koi carp from resident aeromonads.