Detection of psoriasin/S100A7 in the sera of patients with psoriasis
Detection of psoriasin/S100A7 in the sera of patients with psoriasis
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DOI：
10.1111/j.1365-2133.2008.08904.x
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发表时间：
2009-02-01
影响因子：
10.3
通讯作者：
EnerbAck, C.
中科院分区：
文献类型：
--
作者：
Anderson, K. S.;Wong, J.;EnerbAck, C.
银屑病是一种炎症失调和皮肤上皮细胞过度增殖的疾病，涉及先天性和适应性免疫系统。银屑病角质形成细胞表达高水平的银屑病素（S100 A7），一种小的钙结合蛋白。为了确定活动性银屑病患者是否具有升高的血清银屑病素和银屑病素特异性自身抗体水平，从14名寻常型银屑病患者开始窄谱紫外线（UV）B治疗时和从这些患者中的11名患者在UVB治疗过程中每2周收集血液。用夹心酶联免疫吸附试验检测患者和对照血清中银屑病抗原水平，用重组纯化的银屑病蛋白和重叠肽检测银屑病自身抗体滴度，免疫组化证实银屑病表皮中银屑病蛋白有强而特异的表达。患者的全身银屑病抗原水平（平均213 ng/mL）低于对照组（平均331 ng/mL，P = 0.308），并随疾病严重程度的增加而降低。银屑病特异性自身抗体在银屑病患者和健康正常供体的亚组中检测到（平均值0.347 vs. 0.255单位，P = 0.246）。由自身抗体识别的表位被映射到分子的外环结构域，但没有显示出相应的T细胞immunogenicity.Although银屑病在银屑病皮损中过度表达，银屑病的全身水平往往是较低的，随着疾病的严重程度，这可能是由于银屑病特异性自身抗体的存在。无论是银屑病还是银屑病特异性自身抗体似乎都不是临床银屑病的有希望的血清生物标志物。
Psoriasis is a disease of dysregulated inflammation and epithelial hyperproliferation in the skin, involving both the innate and adaptive immune system. Psoriatic keratinocytes express high levels of psoriasin (S100A7), a small calcium-binding protein.To determine if patients with active psoriasis have elevated serum levels of psoriasin and psoriasin-specific autoantibodies.Blood was collected from 14 patients with psoriasis vulgaris at the start of narrowband ultraviolet (UV) B therapy and from 11 of these patients every 2 weeks during the course of the UVB treatment. Patient and control sera were tested for psoriasin antigen levels by sandwich enzyme-linked immunosorbent assay, and for psoriasin autoantibody titres using recombinant purified psoriasin and overlapping peptides.We confirmed strong and specific expression of psoriasin in psoriatic epidermis by immunohistochemistry. Systemic psoriasin antigen levels tended to be lower in patients (mean 213 ng mL(-1)) than in controls (mean 331 ng mL(-1), P = 0.308) and decreased with increasing disease severity. Psoriasin-specific autoantibodies were detected in a subset of patients with psoriasis and healthy normal donors (mean 0.347 vs. 0.255 units, P = 0.246). The epitopes recognized by the autoantibodies were mapped to an external loop domain of the molecule but did not show corresponding T-cell immunogenicity.Although psoriasin is overexpressed in psoriatic skin lesions, systemic levels of psoriasin tended to be lower with increasing disease severity, which may be due to the presence of psoriasin-specific autoantibodies. Neither psoriasin nor psoriasin-specific autoantibodies appear to be promising serum biomarkers for clinical psoriasis.