CPNPG METHYLATION IN MAMMALIAN-CELLS
CPNPG METHYLATION IN MAMMALIAN-CELLS
复制标题
DOI：
10.1038/ng0595-20
复制
发表时间：
1995-05-01
期刊：
影响因子：
30.8
通讯作者：
FROMMER, M
中科院分区：
文献类型：
--
作者：
CLARK, SJ;HARRISON, J;FROMMER, M
在脊椎动物的DNA中，3%到5%的胞嘧啶残基以5-甲基胞嘧啶的形式存在，一般认为，基本上所有的甲基化都发生在对称二核苷酸CpG中包含的胞嘧啶上。在本报告中，我们使用亚硫酸盐基因组测序证明，哺乳动物细胞的甲基化机制能够在CpNpG位点维持和重新甲基化。哺乳动物细胞中存在的遗传性CpNpG甲基化在基因调控和疾病病因学中具有重要意义。
In vertebrate DNA, 3% to 5% of cytosine residues are present as 5-methylcytosine, and it is generally accepted that essentially all of this methylation occurs at cytosines which are contained in the symmetrical dinucleotide CpG. In this report we demonstrate, using bisulphite genomic sequencing, that the methylation machinery of mammalian cells is capable of both maintenance and de novo methylation at CpNpG sites. The existence of inherited CpNpG methylation in mammalian cells has important implications in gene regulation and in the aetiology of disease.