Regulation of antimicrobial peptide expression in human gingival keratinocytes by interleukin-1α
Regulation of antimicrobial peptide expression in human gingival keratinocytes by interleukin-1α
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DOI：
10.1016/j.archoralbio.2011.01.004
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发表时间：
2011-08-01
影响因子：
3
通讯作者：
Kido, Jun-ichi
中科院分区：
文献类型：
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作者：
Hiroshima, Yuka;Bando, Mika;Kido, Jun-ichi
在口腔中，粘膜角质形成细胞部分地通过产生包括防御素、肾上腺髓质素和钙卫蛋白在内的广谱抗菌肽 (AMP) 来抵抗细菌感染。许多 AMP 的表皮角质形成细胞表达随着白细胞介素 1 α (IL-1 α) 的反应而增加。 IL-1 α 由表皮角质形成细胞产生并调节细胞分化。为了更好地了解口腔的先天免疫，我们试图使用 DNA 微阵列和蛋白质印迹分析来确定 IL-1 α 如何调节人牙龈角质形成细胞 (HGK) 的 AMP 表达。来自三名受试者的 HGK 表达 11 种 AMP，包括 S100A7、S100A8、S100A9、S100Al2、分泌性白细胞蛋白酶抑制剂、脂质运载蛋白 2 (LCN2)、半胱氨酸蛋白酶抑制剂 C 和 β-防御素 2。在表达的 AMP 中，S100A7、S100A12 和 LCN2 被 IL-1 α 上调 （诱导型 AMP）；其他 AMP 被认为是本构型的。因此，人牙龈角质形成细胞表达组成型和 IL-1 α 诱导型 AMP，以提供对微生物感染的快速而强大的先天反应。 (C) 2011 Elsevier Ltd. 保留所有权利。
In the oral cavity, mucosal keratinocytes resist bacterial infection, in part, by producing broad-spectrum antimicrobial peptides (AMPs) including defensin, adrenomedullin and calprotectin. Epidermal keratinocyte expression of many AMPs increases in response to interleukin-1 alpha (IL-1 alpha). IL-1 alpha is produced by epidermal keratinocytes and regulates cell differentiation. To better understand innate immunity in the oral cavity, we sought to determine how IL-1 alpha might regulate expression of AMPs by human gingival keratinocytes (HGKs) using DNA microarray and Western blot analyses. HGKs from three subjects expressed eleven AMPs, including S100A7, S100A8, S100A9, S100Al2, secretory leucocyte protease inhibitor, lipocalin 2 (LCN2), cystatin C and beta-defensin 2. Of the expressed AMPs, S100A7, S100Al2 and LCN2 were up-regulated by IL-1 alpha (inducible AMPs); the other AMPs were considered to be constitutive. Human gingival keratinocytes, therefore, express constitutive and IL-1 alpha-inducible AMPs to provide a rapid and robust innate response to microbial infection. (C) 2011 Elsevier Ltd. All rights reserved.