Motility imaging via optical coherence phase microscopy enables label-free monitoring of tissue growth and viability in 3D tissue-engineering scaffolds.
Motility imaging via optical coherence phase microscopy enables label-free monitoring of tissue growth and viability in 3D tissue-engineering scaffolds.
复制标题
通过光学相干相位显微镜进行运动成像，可以对 3D 组织工程支架中的组织生长和活力进行无标记监测。
DOI：
10.1002/term.1687
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发表时间：
2015
影响因子：
3.3
通讯作者：
Holmes C
中科院分区：
文献类型：
--
作者：
Holmes C
随着组织工程领域的不断发展，人们迫切需要无创、无标记的成像技术，以监测厚三维（3D）结构中的组织生长和健康。在研究中的许多成像模式中，光学相干断层扫描（OCT）已成为一种有前途的工具，能够对支架和工程组织进行非破坏性原位表征。然而，使用这种技术的细胞和支架材料之间缺乏光学对比度仍然是一个挑战。在这次交流中，我们表明，映射由细胞活力和运动性引起的光学相位波动允许活细胞与其周围的支架环境的区别。运动成像是通过共路光学相干相位显微镜（OCPM）进行的，这是一种已被证明对纳米级波动敏感的OCT模式。更具体地说，我们检查了两种不同支架系统中人脂肪源性干细胞和/或鼠前成骨细胞的发育，市售藻酸盐海绵和定制微加工聚（d，l-乳酸-共-乙醇酸）纤维支架。细胞运动被证明是OCPM的内源性对比源，能够对3D工程组织发育进行真实的实时、无标记监测。版权所有© 2013约翰威利父子有限公司.
As the field of tissue engineering continues to progress, there is a deep need for non‐invasive, label‐free imaging technologies that can monitor tissue growth and health within thick three‐dimensional (3D) constructs. Amongst the many imaging modalities under investigation, optical coherence tomography (OCT) has emerged as a promising tool, enabling non‐destructivein situcharacterization of scaffolds and engineered tissues. However, the lack of optical contrast between cells and scaffold materials using this technique remains a challenge. In this communication, we show that mapping the optical phase fluctuations resulting from cellular viability and motility allows for the distinction of live cells from their surrounding scaffold environment. Motility imaging was performed via a common‐path optical coherence phase microscope (OCPM), an OCT modality that has been shown to be sensitive to nanometer‐level fluctuations. More specifically, we examined the development of human adipose‐derived stem cells and/or murine pre‐osteoblasts within two distinct scaffold systems, commercially available alginate sponges and custom‐microfabricated poly(d,l‐lactic‐co‐glycolic acid) fibrous scaffolds. Cellular motility is demonstrated as an endogenous source of contrast for OCPM, enabling real‐time, label‐free monitoring of 3D engineered tissue development. Copyright © 2013 John Wiley & Sons, Ltd.