Seed priming with calcium chloride enhances stress tolerance in rice seedlings.
Seed priming with calcium chloride enhances stress tolerance in rice seedlings.
复制标题
DOI：
10.1016/j.plantsci.2022.111381
复制
发表时间：
2022-07
期刊：
影响因子：
--
通讯作者：
Yameng Wang;C. Shen;Qiaochu Jiang;Zhanchun Wang;Chenyang Gao;Wei Wang-
中科院分区：
文献类型：
--
作者：
Yameng Wang;C. Shen;Qiaochu Jiang;Zhanchun Wang;Chenyang Gao;Wei Wang-
钙是植物细胞中重要的第二信使，与植物抵抗生物和非生物胁迫有关。用天然或合成化合物引发植物防御导致更快和更强的抗性反应。然而，用钙预处理植物种子是否可以提高其对胁迫的抵抗力仍然知之甚少。在这项研究中，我们表明，从氯化钙（CaCl 2）预处理种子生长的水稻幼苗表现出增强的抗稻瘟病菌和水稻细菌病原体黄单胞菌。水稻（Xoo）。CaCl 2引发种子还增强了水稻的耐盐性和耐寒性。此外，活性氧（ROS）的爆发显着增加免疫激活水稻幼苗生长从CaCl 2预处理种子。此外，对水稻钙调素结合蛋白60（OsCBP 60）家族进行了分析，发现水稻品种中花11（ZH 11）中存在19个OsCBP 60家族。OsCBP 60的转录产物为几丁质-和M.水稻诱导，这表明他们可能有助于水稻抗性。综上所述，CaCl 2引发可以有效提高水稻种子对多种逆境胁迫的耐受性，其机制可能与促进ROS的爆发有关，OsCBP 60家族成员可能在这一过程中发挥重要作用。
Calcium is a crucial second messenger in plant cells and contributes to plant resistance against biotic and abiotic stress. Plant defense priming with natural or synthetic compounds leads to quicker and stronger resistance responses. However, whether pretreatment of plant seeds with calcium could improve their resistance to stress remains poorly understood. In this study, we showed that rice seedlings grown from calcium chloride (CaCl2)-pretreated seeds displayed enhanced resistance to the rice blast fungus Magnaporthe oryzae and the rice bacterial pathogenXanthomonasoryzaepv.Oryzae(Xoo). Seed priming with CaCl2also led to enhanced rice tolerance to salt and cold. Furthermore, the reactive oxygen species (ROS) burst increased significantly upon immunity activation in the leaves of rice seedlings grown from CaCl2-pretreated seeds. Additionally, we analyzed the rice calmodulin-binding protein 60 (OsCBP60) family and found that there were 19 OsCBP60s in rice cultivar Zhonghua 11 (ZH11). The transcripts of severalOsCBP60s were chitin- andM. oryzae-inducible, suggesting that they may contribute to rice resistance. Taken together, these data indicate that seed priming with CaCl2can effectively enhance rice tolerance to multiple stresses, perhaps by boosting the burst of ROS, and OsCBP60 family members may also play an essential role in this process.