White stripe leaf 12 (WSL12), encoding a nucleoside diphosphate kinase 2 (OsNDPK2), regulates chloroplast development and abiotic stress response in rice (Oryza sativa L.).
White stripe leaf 12 (WSL12), encoding a nucleoside diphosphate kinase 2 (OsNDPK2), regulates chloroplast development and abiotic stress response in rice (Oryza sativa L.).
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白条纹叶 12 (WSL12) 编码核苷二磷酸激酶 2 (OsNDPK2)，调节水稻 (Oryza sativa L.) 的叶绿体发育和非生物胁迫反应
DOI：
10.1007/s11032-016-0479-6
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Guo L
中科院分区：
文献类型：
--
作者：
Ye W;Hu S;Wu L;Ge C;Cui Y;Chen P;Wang X;Xu J;Ren D;Dong G;Qian Q;Guo L
叶绿体是高等植物光合作用和维持正常生命活动的重要细胞器。虽然在水稻中已经鉴定或克隆了一些与叶绿体发育和色素合成相关的基因，但这些基因与非生物胁迫反应之间的关系还知之甚少。在本研究中，我们发现了一个影响水稻色素合成、叶绿体发育和非生物胁迫反应的新突变体--白条叶12(Wsl12)。突变体的表型在苗期和分蘖期以及对温度变化的反应中表现明显。对wsl12与野生型植株正反交的遗传分析表明，wsl12为单核基因隐性突变体。基于图谱的克隆表明，WSL12基因座编码三种核苷二磷酸酶(OsNDPKs)之一的OsNDPK2。WSL12在所有供试组织中都有表达，但在叶片和幼龄组织中高表达。WSL12蛋白定位于叶绿体。Wsl12突变体表现出较高的超氧阴离子水平，增强了对脱落酸(ABA)和盐度的敏感性。在该突变体中，许多参与叶绿素生物合成、ABA合成、光信号途径、清除活性氧途径和另外两个OsNDPKs基因的转录模式发生了变化。这些结果表明，WSL12编码的OsNDPK2基因通过调节相关基因的表达水平，在叶绿体发育和叶绿素生物合成中发挥重要作用。此外，WSL12还影响水稻对非生物胁迫的反应，如ABA和盐度，有利于耐逆分子育种。
Chloroplast is a crucial organelle for plant photosynthesis and maintaining normal life activities in higher plants. Although some genes related to chloroplast development and pigment synthesis have been identified or cloned in rice, little is known about the relationship between these genes and abiotic stress response. In this study, we identified a novel mutantwhite stripe leaf 12(wsl12) affecting pigment synthesis, chloroplast development and abiotic stress response in rice. The mutant phenotype was obvious at seeding and tillering stages and in response to the temperature change. Genetic analysis of reciprocal crosses betweenwsl12and wild-type plants showed thatwsl12was a recessive mutant in a single nuclear locus. Map-based cloning revealed that theWSL12locus encoded OsNDPK2, one of the three nucleoside diphosphate kinases (OsNDPKs).WSL12expressed in all tested tissues, while it highly expressed in leaves and young tissues. The WSL12 protein localized to the chloroplast. Thewsl12mutant showed higher superoxide anion level and enhanced sensitivity to abscisic acid (ABA) and salinity. The transcription pattern of many genes involved in chlorophyll biosynthesis, ABA synthesis, light signaling pathway, reactive oxygen species-scavenging pathway and the other twoOsNDPKswas altered in thewsl12mutant. These results indicate that the OsNDPK2 encoded byWSL12plays an important role in chloroplast development and chlorophyll biosynthesis by regulating the expression levels of related genes. In addition,WSL12also affects the response to abiotic stress, such as ABA and salinity in rice, and is beneficial to molecular breeding of stress tolerance.