Genome-Wide Transcriptomic and Proteomic Exploration of Molecular Regulations in Quinoa Responses to Ethylene and Salt Stress.
Genome-Wide Transcriptomic and Proteomic Exploration of Molecular Regulations in Quinoa Responses to Ethylene and Salt Stress.
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藜麦对乙烯和盐胁迫反应的分子调控的全基因组转录组学和蛋白质组学探索
DOI：
10.3390/plants10112281
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发表时间：
2021-10-25
期刊：
影响因子：
--
通讯作者：
Dong CH
中科院分区：
文献类型：
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作者：
Ma Q;Su C;Dong CH
藜麦（Chenopodium Quinoa Willd.）原产于南美洲安第斯地区，具有较强的耐盐性。为了揭示植物激素乙烯如何参与藜麦对盐胁迫的响应，我们收集了4周龄的‘NL-6’藜麦幼苗，分别用水、氯化钠（NaCl）和含有乙烯前体1-氨基环丙烷-1-羧酸（ACC）的NaCl处理，并通过转录测序和串联质量标签（TMT）定量蛋白质组学分析。共鉴定出9672个蛋白和60602个基因。其中，编码谷胱甘肽s -转移酶（GST）、过氧化物酶（POD）、磷酸盐转运蛋白（PT）、葡聚糖内切酶（GLU）、β -半乳糖苷酶（BGAL）、纤维素合成酶（CES）、毛状体双折光样蛋白（TBL）、富含甘氨酸的细胞壁结构蛋白（GRP）、葡萄糖基转移酶（GT）、GDSL酯酶/脂肪酶（GELP）、细胞色素P450 （CYP）和栀子酸诱导蛋白（JIP）的基因表达差异显著。进一步分析表明，这些基因可能通过渗透调节、细胞壁组织、活性氧清除和植物激素信号等途径参与藜麦对乙烯和盐胁迫的调控。研究结果为探究藜麦对乙烯和盐胁迫的分子机制提供了基础。
Quinoa (Chenopodium quinoa Willd.), originated from the Andean region of South America, shows more significant salt tolerance than other crops. To reveal how the plant hormone ethylene is involved in the quinoa responses to salt stress, 4-week-old quinoa seedlings of ‘NL-6′ treated with water, sodium chloride (NaCl), and NaCl with ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) were collected and analyzed by transcriptional sequencing and tandem mass tag-based (TMT) quantitative proteomics. A total of 9672 proteins and 60,602 genes was identified. Among them, the genes encoding glutathione S-transferase (GST), peroxidase (POD), phosphate transporter (PT), glucan endonuclease (GLU), beta-galactosidase (BGAL), cellulose synthase (CES), trichome birefringence-like protein (TBL), glycine-rich cell wall structural protein (GRP), glucosyltransferase (GT), GDSL esterase/lipase (GELP), cytochrome P450 (CYP), and jasmonate-induced protein (JIP) were significantly differentially expressed. Further analysis suggested that the genes may mediate through osmotic adjustment, cell wall organization, reactive oxygen species (ROS) scavenging, and plant hormone signaling to take a part in the regulation of quinoa responses to ethylene and salt stress. Our results provide a strong foundation for exploration of the molecular mechanisms of quinoa responses to ethylene and salt stress.