Dark dyes-bright complexes: fluorogenic protein labeling.
Dark dyes-bright complexes: fluorogenic protein labeling.
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DOI：
10.1016/j.cbpa.2015.05.014
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发表时间：
2015-08
影响因子：
7.8
通讯作者：
Bruchez MP
中科院分区：
文献类型：
--
作者：
Bruchez MP
有机染料和遗传编码蛋白质之间形成的复合物联合收割机结合了稳定和可调荧光分子以及可靶向的生物整合标记的优点。为了克服用明亮的荧光染料标记所带来的挑战，现在有许多方法利用化学或环境变化来控制结合染料的性质，将染料从弱荧光状态转化为明亮的，易于检测的复合物。优化后，这种方法避免了去除未结合染料的需要，促进了培养细胞中快速简单的测定，并使杂交标记能够在活的模型生物体中更稳健地发挥作用。
Complexes formed between organic dyes and genetically encoded proteins combine the advantages of stable and tunable fluorescent molecules and targetable, biologically integrated labels. To overcome the challenges imposed by labeling with bright fluorescent dyes, a number of approaches now exploit chemical or environmental changes to control the properties of a bound dye, converting dyes from a weakly fluorescent state to a bright, easily detectable complex. Optimized, such approaches avoid the need for removal of unbound dyes, facilitate rapid and simple assays in cultured cells and enable hybrid labeling to function more robustly in living model organisms.