Abnormal taste perception in mice lacking the type 3 inositol 1,4,5-trisphosphate receptor
Abnormal taste perception in mice lacking the type 3 inositol 1,4,5-trisphosphate receptor
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DOI：
10.1074/jbc.m705641200
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发表时间：
2007-12-21
影响因子：
4.8
通讯作者：
Mikoshiba, Katsuhiko
中科院分区：
文献类型：
--
作者：
Hisatsune, Chihiro;Yasumatsu, Keiko;Mikoshiba, Katsuhiko
肌醇1，4，5-三磷酸受体(IP3R)是内质网表达的重要钙通道之一，在多种生理现象中发挥重要作用。3型IP3R在味觉细胞中表达，但该受体在体内味觉知觉中的生理相关性尚不清楚。在这里，我们表明，缺乏IP(3)R3的小鼠表现出对甜、鲜味和苦味物质的异常行为和电生理反应，这些物质触发了G蛋白偶联受体的激活。相比之下，突变小鼠对咸味和酸味的反应基本上是正常的。我们得出结论，IP3R3是甜味、苦味和鲜味知觉的主要中介，可能是连接磷脂酶Cβ2和Trpm5激活的缺失分子。
Inositol 1,4,5-trisphosphate receptor (IP3R) is one of the important calcium channels expressed in the endoplasmic reticulum and has been shown to play crucial roles in various physiological phenomena. Type 3 IP3R is expressed in taste cells, but the physiological relevance of this receptor in taste perception in vivo is still unknown. Here, we show that mice lacking IP(3)R3 show abnormal behavioral and electrophysiological responses to sweet, umami, and bitter substances that trigger G-protein-coupled receptor activation. In contrast, responses to salty and acid tastes are largely normal in the mutant mice. We conclude that IP3R3 is a principal mediator of sweet, bitter, and umami taste perception and would be a missing molecule linking phospholipase C beta 2 to TRPM5 activation.