NITROIMIDAZOLES AS ANAEROBIC ELECTRON-ACCEPTORS FOR XANTHINE-OXIDASE
NITROIMIDAZOLES AS ANAEROBIC ELECTRON-ACCEPTORS FOR XANTHINE-OXIDASE
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DOI：
10.1016/0006-2952(82)90556-1
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发表时间：
1982-01-01
影响因子：
5.8
通讯作者：
WARDMAN, P
中科院分区：
文献类型：
--
作者：
CLARKE, ED;GOULDING, KH;WARDMAN, P
测定了15种硝基咪唑类化合物在黄嘌呤-黄嘌呤氧化酶作用下的厌氧硝化还原速率。所研究的化合物主要是2-硝基咪唑类化合物，包括在癌症治疗中有潜在价值的咪唑类化合物；大多数5-硝基咪唑类化合物，如甲硝唑和尼莫拉唑，在所用条件下反应太慢，无法测量。使用0.1 mM的硝基咪唑，还原速率在.apprx之间变化。4和680nmoL·min~(-1)U~(-1)黄嘌呤氧化物酶。C和pH为7.4。这些速率与表征1-电子还原为硝基自由基阴离子的还原电势相关，并与其他硝基芳基化合物和自由FMN的还原电势相比较。
The rates of anaerobic nitroreduction of 15 nitroimidazoles by xanthine-xanthine oxidase were measured. The compounds studied were mainly 2-nitroimidazoles, including misonidazole and analogs of potential value in cancer therapy; most 5-nitroimidazoles, e.g., metronidazole and nimorazole, reacted too slowly for measurement under the conditions used. Using 0.1 mM nitroimidazole, reduction rates varied between .apprx. 4 and 680 nmol min-1 U-1 xanthine oxidase at 37.degree. C and pH 7.4. These rates were correlated with the reduction potential characterizing 1-electron reduction to the nitro radical-anion, and compared with those for other nitroaryl compounds and with reduction by free FMN.