Conservation of the pyrrolnitrin biosynthetic gene cluster among six pyrrolnitrin-producing strains
Conservation of the pyrrolnitrin biosynthetic gene cluster among six pyrrolnitrin-producing strains
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DOI：
10.1111/j.1574-6968.1999.tb08775.x
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发表时间：
1999-11-01
影响因子：
2.1
通讯作者：
van Pée, KH
中科院分区：
文献类型：
--
作者：
Hammer, PE;Burd, W;van Pée, KH
荧光假单胞菌的punABCD基因簇编码硝基吡啶的生物合成途径，硝基吡啶是色氨酸衍生的次级代谢物，具有很强的抗真菌活性。我们以荧光假单胞菌BL915的双环基因为探针，克隆了另外三株假单胞菌——洋葱伯克霍尔德菌和黄粘球菌的同源基因并进行了测序。除黄毒杆菌的prnA基因外，推断出的氨基酸序列在菌株间相似度为> ~ 59%，表明pyrrolnitrin生物合成的生化途径是高度保守的。黄毒杆菌的prnA基因与其他菌株的prnA相似度约为45%，并且包含在所有6株菌株中高度保守的区域。(C) 1999年欧洲微生物学会联合会。Elsevier Science B.V.版权所有。
The punABCD gene cluster from Pseudomonas fluorescens encodes the biosynthetic pathway for pyrrolnitrin, a secondary metabolite derived from tryptophan which has strong anti-fungal activity. We used the pun genes from P. fluorescens strain BL915 as a probe to clone and sequence homologous genes from three other Pseudomonas strains, Burkholderia cepacia and Myxococcus fulvus. With the exception of the prnA gene from M. fulvus, the deduced amino acid sequences were > 59% similar among the strains, indicating that the biochemical pathway for pyrrolnitrin biosynthesis is highly conserved. The prnA gene from M. fulvus is about 45% similar to prnA from the other strains and contains regions which are highly conserved among all six strains. (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.