Optimising the construction of a substitution library in Arabidopsis thaliana using computer simulations
Optimising the construction of a substitution library in Arabidopsis thaliana using computer simulations
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DOI：
10.1023/b:molb.0000012845.37366.b5
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发表时间：
2004-01-01
影响因子：
3.1
通讯作者：
Kearsey, MJ
中科院分区：
文献类型：
--
作者：
Syed, NH;Pooni, HS;Kearsey, MJ
利用计算机模拟研究了拟南芥（Arabidopsis thaliana） Landsberg erecta （Ler）和Columbia （Col）生态型之间构建重叠部分染色体替代文库的可行性。这样的文库可以有效地用于该物种数量性状位点（qtl）的精细定位，将Col的供体束插入到Ler的循环背景中，反之亦然。这项研究是基于已知的Ler和Col生态型的RFLP谱，以及一些已经通过自花授粉从杂交中提取的重组自交系（RILs）的RFLP谱。结果表明，只要将11个ril与其各自的复发/受体亲本杂交，并从F-2或Bc(1)代或其自生后代中选择所需的重组基因，就可以在基因组的很大一部分（约80%）上产生纯合子替代系。然而，在四个ril的情况下，至少需要进行两轮回交，以便在自交/选择之前从背景基因型中去除不需要的供体束。模拟还提供了有关育种计划规模的宝贵信息，并表明在4-5代的短时间内可以产生多达59个替代资源。
The feasibility of constructing an overlapping part-chromosome substitution library between the Landsberg erecta (Ler) and Columbia (Col) ecotypes of Arabidopsis thaliana was investigated using computer simulations. Such a library can be used effectively in the fine mapping of quantitative trait loci (QTLs) in this species, with donor tracts of Col inserted into the recurrent background of Ler and vice versa. The study was based on the known RFLP profiles of Ler and Col ecotypes, and of some selected recombinant inbred lines (RILs) that have already been extracted from their cross by self pollination. It was shown that homozygous substitution lines can be produced for a large segment of the genome ( > 80%) by crossing just 11 RILs with their respective recurrent/recipient parents and selecting the desired recombinants from the F-2 or Bc(1) generation or their selfed progenies. In the case of four RILs, however, at least two rounds of backcrossing were deemed necessary to remove the unwanted donor tracts from the background genotype prior to selfing/selection. The simulations also provided valuable information on the scale of the breeding programme and show that a resource of up to 59 substitutions can be produced within a short period of 4-5 generations.