In vitro effects of cationic compounds on functional human sperm parameters
In vitro effects of cationic compounds on functional human sperm parameters
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DOI：
10.1016/j.fertnstert.2012.11.008
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发表时间：
2013-03-01
影响因子：
6.7
通讯作者：
Ramalho-Santos, Joao
中科院分区：
文献类型：
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作者：
Baptista, Marta;Publicover, Stephen J.;Ramalho-Santos, Joao
目的：确定阳离子化合物杀精子活性的机制。设计：人类精子的体外研究。设置：与大学医院合作的学术研究机构。患者：正常精子的男性，为常规分析或辅助生殖技术提供样本。干预：用市售阳离子表面活性剂体外孵育精子。主要结果测量：监测苯扎溴铵（C（12）Bzk）、溴化吡啶（C（12）Pyr）和十二烷基三甲基溴化铵（C（12）TAB）对人精子活力、运动力、线粒体状态、获能、顶体状态和钙运动的影响。180分钟后，线粒体膜电位普遍降低。用C（12）Bzk孵育180分钟后，在LD 50下，精子获能较少（46.0%），20.2%的细胞显著增加其细胞内钙。C（12）Pyr是较强的化合物，在LD 50时影响获能和顶体状态，并促进较高的钙内流（81.7%）。C（12）TAB在LD 50作用180 min后可增加顶体反应的精子数，并导致钙信号的显著增加（分别为61.2%和67.9%）。结论：阳离子表面活性剂似乎在不同程度上损害精子功能，但C（12）Pyr更有效。考虑到使用的浓度，这些化合物不一定充当表面活性剂。据我们所知，这是第一项通过监测精子钙信号来评估避孕化合物的研究，这似乎是确定假定杀精子剂作用的敏感参数。（Fertil Steril（R）2013; 99：705-12. (C)2013年美国生殖医学会。）
Objective: To determine the mechanisms of spermicidal activity by cationic compounds.Design: In vitro study with human sperm.Setting: Academic research institute in collaboration with a university hospital.Patient(s): Normozoospermic men providing samples for routine analysis or assisted reproductive technologies.Intervention(s): In vitro incubation of sperm with commercially available cationic surfactants.Main Outcome Measure(s): The effects of benzalkonium bromide (C(12)Bzk), pyridinium bromide (C(12)Pyr), and dodecyl trimethylammonium bromide (C(12)TAB) on human sperm viability, motility, mitochondrial status, capacitation, acrosomal status, and calcium movements were monitored.Result(s): There was a universal decrease in mitochondrial membrane potential after 180 minutes. Incubation with C(12)Bzk led to less capacitated sperm at LD50 after 180 minutes (46.0%) as well as to 20.2% of cells significantly increasing their intracellular calcium. C(12)Pyr was the stronger compound, affecting capacitation and acrosomal status at LD50 and promoting a higher calcium influx (81.7%). C(12)TAB increased the number of acrosome-reacted sperm at LD50 after 180 minutes and led to a significant increase in calcium signaling (61.2% and 67.9%, respectively).Conclusion(s): Cationic surfactants seem to impair sperm function at various levels, although C(12)Pyr was shown to be more effective. Given the concentrations used, these compounds are not necessarily acting as surfactants. To our knowledge, this is the first study that evaluates contraceptive compounds by monitoring sperm calcium signaling, and this seems to be a sensitive parameter in terms of determining the action of putative spermicides. (Fertil Steril (R) 2013; 99:705-12. (C) 2013 by American Society for Reproductive Medicine.)