CLONING AND CHARACTERIZATION OF A cDNA ENCODING A SEXUAL CELL DIVISION‐INDUCING PHEROMONE FROM A UNICELLULAR GREEN ALGA CLOSTERIUM EHRENBERGII (CHLOROPHYTA)
CLONING AND CHARACTERIZATION OF A cDNA ENCODING A SEXUAL CELL DIVISION‐INDUCING PHEROMONE FROM A UNICELLULAR GREEN ALGA CLOSTERIUM EHRENBERGII (CHLOROPHYTA)
复制标题
编码来自单细胞绿藻新月藻（绿藻）性细胞分裂诱导信息素的 cDNA 的克隆和表征
DOI：
10.1046/j.1529-8817.2003.03004.x
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发表时间：
2003
影响因子：
2.9
通讯作者：
H. Sekimoto
中科院分区：
文献类型：
--
作者：
R. Fukumoto;T. Fujii;H. Sekimoto
从一种异thalic绿藻Closterium ehrenbergii Menegh中分离到了一个编码性细胞分裂诱导信息素（SCD‐IP）的全长cDNA。举例来说，采用cDNA末端快速扩增- PCR方法。该cDNA全长907 bp，编码一个含有209个氨基酸的蛋白，分子量为23186。预测的蛋白有一个可能的糖基化位点和一个用于细胞外分泌的疏水信号肽。SCD‐IP基因的转录本仅在交配型阴性（mt -）细胞中检测到，当mt -细胞与交配型阳性（mt+）细胞共培养时，转录本显著增加。相比之下，SCD‐IP基因在两种交配型细胞中均作为多拷贝基因被Southern blot检测到。持续的黑暗条件和培养基中氮源的补充抑制了SCD - IP的表达。从另一种Closterium物种中分离出的性信息素SCD - IP和原生质体释放诱导蛋白（PR - IP）诱诱剂的基因编码的氨基酸序列似乎具有显著的同源性，这表明这些性信息素是从单一的Closterium祖先信息素中分化出来的。
A full‐length cDNA encoding a sexual cell division‐ inducing pheromone (SCD‐IP) was isolated from a heterothallic green alga, Closterium ehrenbergii Menegh. ex Ralfs, using the rapid amplification of cDNA ends‐PCR method. The cDNA was 907 bp in length and coded for a protein of 209 amino acids with a deduced molecular mass of Mr 23,186. The predicted protein had a possible glycosylation site and a hydrophobic signal peptide for extracellular secretion. Transcripts of the SCD‐IP gene were detected only in mating type minus (mt−) cells and remarkably increased when mt− cells were cocultured with mating type plus (mt+) cells. By contrast, the gene for SCD‐IP was detected in both mating type cells as multicopy genes by Southern blot analysis. Continuous dark conditions and supplementation of a nitrogen source to the culture medium suppressed SCD‐IP expression. The deduced amino acid sequences encoded by genes for SCD‐IP and protoplast‐release‐inducing protein (PR‐IP) Inducer, a sex pheromone isolated from another Closterium species, appeared to share significant homology, suggesting that these sex pheromones were diversified from a single ancestral Closterium pheromone.
异戊二烯蛋白蛋白酶在酿酒酵母 a 因子成熟中的作用。
影响因子：
3.3
作者：
Boyartchuk,VL;Rine,J
通讯作者：
Rine,J