Pfs 16 pivotal role in Plasmodium falciparum gametocytogenesis: A potential antiplasmodial drug target
Pfs 16 pivotal role in Plasmodium falciparum gametocytogenesis: A potential antiplasmodial drug target
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DOI：
10.1016/j.exppara.2008.10.010
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发表时间：
2009-02-01
影响因子：
2.1
通讯作者：
Benoit-Vical, Francoise
中科院分区：
文献类型：
--
作者：
Berry, Antoine;Deymier, Caroline;Benoit-Vical, Francoise
成熟的配子母细胞，即恶性疟原虫的有性阶段，确保了疟疾从人类宿主向蚊子媒介的持续传播。即使配子体与疟疾的病理生理学无关，它对疟疾的传播也至关重要。在公共卫生领域，配子细胞将成为抗疟原虫药物的关键靶点。应用真实的时间PCR技术，分析了恶性疟原虫配子体发生过程中Pfs 16、Pfs 25、Pfg 27和S18S rRNA 4个性期特异基因的表达水平。菌株表现出不同的能力，以产生成熟的配子体和平行的不同模式的性基因表达。Pfs 16 cDNA在培养的第一个48小时过表达与成熟配子体的产生之间存在相关性。Pfs 16是培养物中成熟配子母细胞发育的早期标志物，因此是新抗疟药物的潜在靶点。(C)2008年爱思唯尔公司All rights reserved.
Mature gametocytes, the sexual stage of Plasmodium falciparum, ensure the continued transmission of malaria from the human host to the mosquito vector. Even if gametocytes are not implicated in the malaria physiopathology it is crucial to the spread of malaria. Gametocytes are to be a key target for drugs used against Plasmodium in public health. The expression levels of 4 sexual-stage specific genes, Pfs 16, Pfs 25, Pfg 27and S 18S rRNA, during gametocytogenesis of various P. falciparum strains were analyzed by a real time PCR assay. The strains showed different capacities to produce mature gametocytes and in parallel different patterns of sexual gene expression. There was a correlation only between Pfs 16 cDNA overexpression in the first 48 h of the culture and the production of mature gametocytes. Pfs 16 is an early marker of the development of mature gametocytes in cultures and is therefore a potential target for new antimalarial drugs. (C) 2008 Elsevier Inc. All rights reserved.