A mitochondrial protein homologous to the mammalian peripheral-type benzodiazepine receptor is essential for stress adaptation in plants
A mitochondrial protein homologous to the mammalian peripheral-type benzodiazepine receptor is essential for stress adaptation in plants
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DOI：
10.1111/j.1365-313x.2007.03198.x
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发表时间：
2007-09-01
期刊：
影响因子：
7.2
通讯作者：
Reski, Ralf
中科院分区：
文献类型：
--
作者：
Frank, Wolfgang;Baar, Kim-Miriam;Reski, Ralf
从苔藓小立碗藓中克隆非生物胁迫诱导基因，鉴定了基因PpTSPO1，该基因编码一种与哺乳动物线粒体外周型苯二氮卓受体和细菌富含色氨酸的感觉蛋白同源的蛋白。这类蛋白质参与四吡咯生物合成途径中间体的转运。与哺乳动物同源物一样，PpTSPO1蛋白定位于线粒体。PpTSPO1靶向的苔藓敲除株系的产生揭示了该基因在非生物胁迫适应中的重要功能。在胁迫条件下，PpTSPO1无效突变体显示H2O2水平升高，脂质过氧化和细胞死亡增强，表明PpTSPO1在氧化还原稳态中的重要作用。我们推测，PpTSPO1的行为直接卟啉前体的血红素形成的线粒体，并参与光反应性四吡咯中间体的去除。
The cloning of abiotic stress-inducible genes from the moss Physcomitrella patens led to the identification of the gene PpTSPO1, encoding a protein homologous to the mammalian mitochondrial peripheral-type benzodiazepine receptor and the bacterial tryptophane-rich sensory protein. This class of proteins is involved in the transport of intermediates of the tetrapyrrole biosynthesis pathway. Like the mammalian homologue, the PpTSPO1 protein is localized to mitochondria. The generation of PpTSPO1-targeted moss knock-out lines revealed an essential function of the gene in abiotic stress adaptation. Under stress conditions, the PpTSPO1 null mutants show elevated H2O2 levels, enhanced lipid peroxidation and cell death, indicating an important role of PpTSPO1 in redox homeostasis. We hypothesize that PpTSPO1 acts to direct porphyrin precursors to the mitochondria for heme formation, and is involved in the removal of photoreactive tetrapyrrole intermediates.