Inositol 1,4,5 trisphosphate releases calcium from specialized sites within Limulus photoreceptors.
Inositol 1,4,5 trisphosphate releases calcium from specialized sites within Limulus photoreceptors.
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DOI：
10.1083/jcb.104.4.933
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发表时间：
1987-04
期刊：
影响因子：
--
通讯作者：
Fein A
中科院分区：
文献类型：
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作者：
Payne R;Fein A
我们已经调查了亚细胞分布和身份的三磷酸肌醇（InsP3）敏感的钙存储在生活鲎腹侧感光细胞，其中光和InsP3是已知的提高细胞内钙。我们将发光蛋白水母发光蛋白注入腹侧感光细胞，并用图像增强器观察其发光。InsP3仅在注射到光敏横纹肌（R）叶中时引起可检测的水母发光，其中光诱导的水母发光也被限制。因此，由光和InsP3释放的钙储存定位于R叶。在R叶内，由于注射的InsP3的快速稀释和/或降解，InsP3诱导的水母发光蛋白发光被进一步限制在注射部位周围。滑面内质网的突出池独特地位于R叶的微绒毛表面下方的细胞内（Calman，B.，和s. Chamberlain，1982，生理学杂志，80：839 - 862）。这些脑池可能是InsP3的作用部位。
We have investigated the subcellular distribution and identity of inositol trisphosphate (InsP3)-sensitive calcium stores in living Limulus ventral photoreceptor cells, where light and InsP3 are known to raise intracellular calcium. We injected ventral photoreceptor cells with the photoprotein aequorin and viewed its luminescence with an image intensifier. InsP3 only elicited detectable aequorin luminescence when injected into the light-sensitive rhabdomeral (R)-lobe where aequorin luminescence induced by light was also confined. Calcium stores released by light and InsP3 are therefore localized to the R- lobe. Within the R-lobe, InsP3-induced aequorin luminescence was further confined around the injection site, due to rapid dilution and/or degradation of injected InsP3. Prominent cisternae of smooth endoplasmic reticulum are uniquely localized within the cell beneath the microvillar surface of the R-lobe (Calman, B., and S. Chamberlain, 1982, J. Gen. Physiol., 80:839-862). These cisternae are the probable site of InsP3 action.