Genome-wide identification of the maize 2OGD superfamily genes and their response to Fusarium verticillioides and Fusarium graminearum
Genome-wide identification of the maize 2OGD superfamily genes and their response to Fusarium verticillioides and Fusarium graminearum
复制标题
玉米 2OGD 超家族基因的全基因组鉴定及其对轮枝镰刀菌和禾谷镰刀菌的响应
DOI：
10.1016/j.gene.2020.145078
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发表时间：
2021-01-05
期刊：
影响因子：
3.5
通讯作者：
Wang, Guan-Feng
中科院分区：
文献类型：
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作者：
Ge, Chunxia;Tang, Changxiao;Wang, Guan-Feng
在玉米上，由镰刀菌和禾谷镰刀菌引起的腐烂和秸秆腐烂会导致霉粒的污染，从而产生真菌毒素。鉴定玉米抗病基因是玉米抗病育种的有效途径。几种依赖于2-氧戊二酸的双加氧酶(2OGD)蛋白在不同的植物物种中被发现对不同的病原菌具有抗性。然而，人们对玉米中的2OGD超家族知之甚少。在这里，我们从全基因组分析中鉴定了103个在玉米中可能存在的2OGD基因，并将其分为DOXA、DOXB和DOXC三类。我们进一步对它们的基因结构、染色体分布、系统发育树、基因功能丰富以及不同组织间的表达谱进行了全面的研究。编码3种2OGD蛋白的基因ACO、F3H和NCS分别参与乙烯生物合成、黄酮类生物合成和生物碱生物合成途径，分别由轮叶镰刀菌和禾谷镰刀菌诱导。这三个基因的启动子含有转录因子ZmDOF和ZmHSF的结合位点，这两个转录因子也是由这两种病原体诱导的。这些结果表明，这三个2OGDS和两个转录因子可能参与了对这两种病原菌的抗性。本研究对玉米2OGD超家族有了全面的了解，为进一步研究其在玉米抗食腐病和抗茎腐病中的作用奠定了基础。
In maize, eat rot and stalk rot caused by Fusarium verticillioides and Fusarium graminearum lead to contamination of moldy grains to produce mycotoxins. Identification of resistance genes against these pathogens for maize breeding is an effective way for disease control. Several 2-oxoglutarate-dependent dioxygenase (2OGD) proteins have been found to confer resistance to different pathogens in diverse plant species. However, little is known about the 2OGD superfamily in maize. Here, we identified 103 putative 2OGD genes in maize from a genome-wide analysis, and divided them into three classes - DOXA, DOXB, and DOXC. We further comprehensively investigated their gene structure, chromosome distribution, phylogenetic tree, gene-function enrichment, and expression profiles among different tissues. The genes encoding three 2OGD proteins, ACO, F3H, and NCS involved in ethylene biosynthesis, flavonoids biosynthesis, and alkaloids biosynthesis pathways, respectively, were identified to be induced by F. verticillioides and F. graminearum. The promoters of the three genes contain the binding sites for the transcription factor ZmDOF and ZmHSF, which are also induced by the two pathogens. The results imply that the three 2OGDs and the two transcription factors might be involved in the resistance to the two pathogens. This study provided a comprehensive understanding of the 2OGD superfamily in maize and laid the foundation for the further functional analysis of their roles in maize resistance to eat rot and stalk rot.