Integration of Multiplex Bead Assays for Parasitic Diseases into a National, Population-Based Serosurvey of Women 1539 Years of Age in Cambodia
Integration of Multiplex Bead Assays for Parasitic Diseases into a National, Population-Based Serosurvey of Women 1539 Years of Age in Cambodia
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DOI：
10.1371/journal.pntd.0004699
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发表时间：
2016-05-01
影响因子：
3.8
通讯作者：
Lammie, Patrick J.
中科院分区：
文献类型：
--
作者：
Priest, Jeffrey W.;Jenks, M. Harley;Lammie, Patrick J.
监测数据的收集对于监测和评估公共卫生项目至关重要。目前，许多国家通过人口健康调查和多指标调查定期进行以家庭为基础的综合健康数据收集，这为衡量提供保健服务方面的进展提供了重要尺度。相比之下，生物标志物调查通常侧重于疟疾感染、艾滋病毒状况、疫苗接种覆盖率或疫苗可预防疾病（VPD）免疫状况的单一或相关指标。在这里，我们描述了一个综合的生物标志物调查的基础上使用的多重珠检测（MBA），同时测量抗体反应的多种寄生虫病的公共卫生重要性的VPD血清学调查在柬埔寨的一部分。一个具有全国代表性的基于集群的调查，收集血清样本的育龄妇女。通过MBA检测样本中识别恶性疟原虫和间日疟原虫、班氏吴策线虫、弓形虫、猪带绦虫和粪类圆线虫重组抗原的免疫球蛋白G抗体。血清学IgG抗体的结果是有用的，既产生国家流行率估计的利益，并确认高度集中的分布，这些感染的寄生虫病。综合调查为系统评估多个公共卫生方案的状况和衡量实现千年发展目标的进展提供了机会。
Collection of surveillance data is essential for monitoring and evaluation of public health programs. Integrated collection of household-based health data, now routinely carried out in many countries through demographic health surveys and multiple indicator surveys, provides critical measures of progress in health delivery. In contrast, biomarker surveys typically focus on single or related measures of malaria infection, HIV status, vaccination coverage, or immunity status for vaccine-preventable diseases (VPD). Here we describe an integrated biomarker survey based on use of a multiplex bead assay (MBA) to simultaneously measure antibody responses to multiple parasitic diseases of public health importance as part of a VPD serological survey in Cambodia. A nationally-representative cluster-based survey was used to collect serum samples from women of child-bearing age. Samples were tested by MBA for immunoglobulin G antibodies recognizing recombinant antigens from Plasmodium falciparum and P. vivax, Wuchereria bancrofti, Toxoplasma gondii, Taenia solium, and Strongyloides stercoralis. Serologic IgG antibody results were useful both for generating national prevalence estimates for the parasitic diseases of interest and for confirming the highly focal distributions of some of these infections. Integrated surveys offer an opportunity to systematically assess the status of multiple public health programs and measure progress toward Millennium Development Goals.