Agrobacterium-mediated transformation and inbred line development in the model grass Brachypodium distachyon
Agrobacterium-mediated transformation and inbred line development in the model grass Brachypodium distachyon
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DOI：
10.1007/s11240-005-9023-9
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发表时间：
2006-02-01
影响因子：
3
通讯作者：
Hayden, DM
中科院分区：
文献类型：
--
作者：
Vogel, JP;Garvin, DF;Hayden, DM
二穗短柄草 (Brachypodium) 已被提议作为温带草的模型，因为它的物理、遗传和基因组属性（身材小、生长要求简单、基因组大小小、二倍体生态型的可用性、年生命周期和自交）适合模型植物系统。在短柄草被广泛接受为模型系统之前，还需要两个额外的要求：高效的转化系统和同质近交参考基因型。在这里，我们描述了 27 个短柄草属品种的自交系的发育。 c 值的测定表明，五个来源种质是二倍体。这些二倍体品系表现出多种形态特征的变异。确定了允许二倍体产生时间长达两个月的条件。开发了农杆菌介导的转化方案，并用于成功转化所测试的 19 个品系中的 10 个，效率范围为 0.4% 至 15%。二倍体种质Bd21很容易转化。 T-1 代中转基因的分离表明，大多数品系在单个基因位点处包含插入。这里报道的新资源和方法将促进短柄草作为禾本科基因组学新模型系统的开发和利用。
Brachypodium distachyon (Brachypodium) has been proposed as a model temperate grass because its physical, genetic, and genome attributes (small stature, simple growth requirements, small genome size, availability of diploid ecotypes, annual lifecycle and self fertility) are suitable for a model plant system. Two additional requirements that are necessary before Brachypodium can be widely accepted as a model system are an efficient transformation system and homogeneous inbred reference genotypes. Here we describe the development of inbred lines from 27 accessions of Brachypodium. Determination of c-values indicated that five of the source accessions were diploid. These diploid lines exhibit variation for a variety of morphological traits. Conditions were identified that allow generation times as fast as two months in the diploids. An Agrobacterium-mediated transformation protocol was developed and used to successfully transform 10 of the 19 lines tested with efficiencies ranging from 0.4% to 15%. The diploid accession Bd21 was readily transformed. Segregation of transgenes in the T-1 generation indicated that most of the lines contained an insertion at a single genetic locus. The new resources and methodologies reported here will advance the development and utilization of Brachypodium as a new model system for grass genomics.