Prevalence and characteristics of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae isolated from rural well water in Taian, China, 2014
Prevalence and characteristics of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae isolated from rural well water in Taian, China, 2014
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DOI：
10.1007/s11356-015-4387-9
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发表时间：
2015-08-01
影响因子：
5.8
通讯作者：
Chang, Weishan
中科院分区：
文献类型：
--
作者：
Zhang, Hongna;Zhou, Yufa;Chang, Weishan
产超广谱β-内酰胺酶（ESBL）是肠杆菌科细菌的主要耐药机制之一，近年来从水体中分离的产ESBL肠杆菌科细菌数量不断增加，已对公众健康构成严重威胁。目的分析泰安市农村沃茨中产ESBL肠杆菌科细菌的流行特征。共检测到10株表达ESBL表型的菌株，包括9株大肠埃希菌（E. coli）和肺炎克雷伯氏菌（K. pneumoniae.肺炎菌）从100个取样的威尔斯孔中的4个（4%）获得。ESBL基因型显示9例CTX-M-15阳性，1例CTX-M-27阳性。8株产ESBL E.大肠杆菌CTX-M-15属于ST 10型，在我国主要从人粪便中检出。重要的是，CTX-M-27的唯一产生菌株E.大肠杆菌属于多位点序列型B2：131（ST 131），可能与人畜严重感染有关。
The production of extended-spectrum beta-lactamase (ESBL) is one of the major antimicrobial resistance mechanisms in Enterobacteriaceae, and the increasing number of ESBL-producing Enterobacteriaceae isolated from water environments has posed a serious threat to the public health. The study aimed to analyze prevalence and characterization of ESBL-producing Enterobacteriaceae from rural well waters in Taian, China. A total of 10 isolates expressing an ESBL phenotype, including 9 Escherichia coli (E. coli) and 1 Klebsiella pneumoniae (K. pneumoniae) was obtained from 4 (4 %) out of the 100 sampled wells. ESBL genotype revealed that 9 expressed CTX-M-15 and 1 produced CTX-M-27. Five out of 8 ESBL-producing E. coli expressing CTX-M-15 belonged to ST10, which are mostly detected from human feces in China. Importantly, the only strain of CTX-M-27-producing E. coli belonged to multi-locus sequence type B2:131 (ST131), which may be related with severe infection in humans and animals.