The AHK4 gene involved in the cytokinin-signaling pathway as a direct receptor molecule in Arabidopsis thaliana
The AHK4 gene involved in the cytokinin-signaling pathway as a direct receptor molecule in Arabidopsis thaliana
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DOI：
10.1093/pcp/pce094
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发表时间：
2001-07-01
影响因子：
4.9
通讯作者：
Tabata, S
中科院分区：
文献类型：
--
作者：
Ueguchi, C;Sato, S;Tabata, S
我们以前确定了一组结构相关的基因，AHK2，3和4，每个编码传感器组氨酸激酶在拟南芥。为了确定相关的生物学功能，我们鉴定了AHK4基因的功能丧失突变。该突变体不仅表现出抗细胞分裂素的表型，而且在愈伤组织的绿化和芽诱导方面也表现出抗细胞分裂素的表型。此外，在芽殖酵母中表达的AHK 4在细胞分裂素存在的情况下表现出组氨酸激酶活性，这些结果强烈地表明，AHK 4作为直接受体分子参与了拟南芥中细胞分裂素信号传导途径。
We previously identified a set of structurally related genes, AHK2, 3 and 4, each encoding a sensor histidine kinase in Arabidopsis thaliana. To determine the relevant biological functions, we identified a loss-of-function mutation of the AHK4 gene. The mutant exhibited the cytokinin-resistant phenotype not only in inhibition of root growth by cytokinin but also in greening and shoot induction of calli, Moreover, AHK4 expressed in budding yeast showed histidine kinase activity in a manner dependent on the presence of cytokinin, These results strongly suggested that AHK4 is involved in the cytokinin-signaling pathway, as a direct receptor molecule, in Arabidopsis.