Proteomic profiling of plasma biomarkers in acute ischemic stroke due to large vessel occlusion
Proteomic profiling of plasma biomarkers in acute ischemic stroke due to large vessel occlusion
复制标题
大血管闭塞引起的急性缺血性中风血浆生物标志物的蛋白质组学分析
DOI：
10.1186/s12967-019-1962-8
复制
发表时间：
2019-07-01
影响因子：
7.4
通讯作者：
Tian, Dai-Shi
中科院分区：
文献类型：
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作者：
Qin, Chuan;Zhao, Xin-Ling;Tian, Dai-Shi
大血管闭塞（LVO）引起的急性缺血性脑卒中（AIS）是一种严重的脑血管疾病，侧支循环是其治疗的重要手段。与急性LVO发病机制和内皮功能相关的蛋白质可能会出现在AIS患者的血液样本中，由于LVO，从而允许发展血液为基础的生物标志物，其诊断和预后。招募2016年7月至2018年4月在同济医院神经内科就诊的并发症患者。在发现阶段，使用基于iTRAQ的LC-MS/MS的蛋白质组学方法研究了LVO所致AIS患者血浆中蛋白质组模式的改变。在验证研究中，蛋白质印迹法被用来确定与中风诊断相关的生物标志物，以及与不同的侧支status.ResultsFor这项探索性研究，血浆蛋白质组学分析40例AIS由于LVO和20名健康对照组显示7个差异表达的蛋白质组之间的差异为1.2/0.83倍或更大的差异。PPBP（1.58 ± 0.78 vs 0.98 ± 0.37; P
BackgroundAcute ischemic stroke (AIS) due to large vessel occlusion (LVO) is a devastating cerebrovascular disorder, which could benefit from collateral circulation. Proteins associated with acute LVO pathogenesis and endothelial function may appear in blood samples of AIS patients due to LVO, thus permitting development of blood-based biomarkers for its diagnosis and prognosis.MethodsThis study is a single-center, retrospective, observational case-control trial. Consecutive patients who presented at the Department of Neurology of Tongji Hospital were recruited from July 2016 to April 2018. In the discovery phase, a proteomic approach with iTRAQ-based LC-MS/MS was used to investigate the altered proteomic pattern in plasma from patients with AIS due to LVO. In the validation study, Western blots was used to identify biomarkers associated with stroke diagnosis as well as their prognostic value associated with different collateral statuses.ResultsFor this exploratory study, the proteomic analysis of plasma from 40 patients with AIS due to LVO and 20 healthy controls revealed seven differentially expressed proteins with a 1.2/0.83-fold or greater difference between groups. The four elevated proteins, PPBP (1.580.78 vs 0.980.37; P