Novel large-scale chromosomal transfer in Bacteroides fragilis contributes to its pan-genome and rapid environmental adaptation.
Novel large-scale chromosomal transfer in Bacteroides fragilis contributes to its pan-genome and rapid environmental adaptation.
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DOI：
10.1099/mgen.0.000136
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发表时间：
2017-11
影响因子：
3.9
通讯作者：
Wexler HM
中科院分区：
文献类型：
--
作者：
Husain F;Tang K;Veeranagouda Y;Boente R;Patrick S;Blakely G;Wexler HM
脆弱拟杆菌是人体胃肠道菌群的重要组成部分，一旦从胃肠道逃逸，可引起致命的肠外感染。我们证明了来自B的大染色体片段的转移和重组。多药耐药临床分离株fragilis HMW 615对B的耐药性较好。fragilis 638R.在一个实例中，~435 KB/356基因的区段的转移替换了B的~413 KB/326基因。fragilis 638R染色体。除了抗生素抗性基因的转移之外，这些转移（1）替换完全趋异的多糖生物合成基因座;（2）用更复杂的趋异的shufflons替换DNA倒位控制的基因间shufflons（其控制编码淀粉利用系统外膜蛋白的基因的表达）;以及（3）引入额外的编码趋异的1型限制/修饰系统的基因间shufflons。转移片段内的接合转座子样基因和转移片段下游约45 kb的推定整合接合元件（ICE 5）内的接合转座子样基因都编码可能参与观察到的转移的蛋白质。这些数据表明，染色体转移是类杆菌抗原多样性和营养适应的驱动因素，（1）有助于广泛的B的传播。fragilis泛基因组，（2）允许快速适应不断变化的环境和（3）可以赋予宿主共生体致病特性。
Bacteroides fragilis, an important component of the human gastrointestinal microbiota, can cause lethal extra-intestinal infection upon escape from the gastrointestinal tract. We demonstrated transfer and recombination of large chromosomal segments from B. fragilis HMW615, a multidrug resistant clinical isolate, to B. fragilis 638R. In one example, the transfer of a segment of ~435 Kb/356 genes replaced ~413 Kb/326 genes of the B. fragilis 638R chromosome. In addition to transfer of antibiotic resistance genes, these transfers (1) replaced complete divergent polysaccharide biosynthesis loci; (2) replaced DNA inversion-controlled intergenic shufflons (that control expression of genes encoding starch utilization system outer membrane proteins) with more complex, divergent shufflons; and (3) introduced additional intergenic shufflons encoding divergent Type 1 restriction/modification systems. Conjugative transposon-like genes within a transferred segment and within a putative integrative conjugative element (ICE5) ~45 kb downstream from the transferred segment both encode proteins that may be involved in the observed transfer. These data indicate that chromosomal transfer is a driver of antigenic diversity and nutrient adaptation in Bacteroides that (1) contributes to the dissemination of the extensive B. fragilis pan-genome, (2) allows rapid adaptation to a changing environment and (3) can confer pathogenic characteristics to host symbionts.
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影响因子：
3.6
作者：
Daccord, Aurelie;Ceccarelli, Daniela;Burrus, Vincent
通讯作者：
Burrus, Vincent
影响因子：
4.9
作者：
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Wexler, Hannah M.
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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