Assembly and disassembly of the Golgi complex: two processes arranged in a cis-trans direction
Assembly and disassembly of the Golgi complex: two processes arranged in a cis-trans direction
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高尔基复合体的组装和拆卸：顺反方向排列的两个过程
作者：
J. Alcalde;P. Bonay;A. Roa;S. Vilaró;I. Sandoval
我们已经研究了拆卸和组装的两个形态和功能不同的部分高尔基复合体，顺式/中间和反式池/反式网络隔间。为了这个目的，我们遵循的重新分配的顺式/中间-（GMPc-1，GMPc-2，MG 160）和两个反式-（GMPt-1和GMPt-2）高尔基膜蛋白的过程中和处理后的正常大鼠肾（NRK）细胞与布雷菲德菌素A（BFA）。BFA诱导顺式/中间-和反式-高尔基体复合体的完全解体以及GMPc和GMPt向ER的易位。短时间（3分钟）与BFA处理的细胞表现出广泛的解体的顺式/中间和反式高尔基复合体。然而，反式部分的完全解体需要更长的药物孵育时间。去除BFA后，高尔基复合体经三个步骤重组：（a）内质网中的顺式/中间蛋白存在并聚集成囊泡状结构，（B）反式蛋白在含有顺式/中间蛋白的囊泡中逐渐迁移和聚集;和（c）在位于中心体附近的区域内组装池，并重建高尔基复合体，其中ER被排除在外。顺式/中间高尔基复合体的重建发生在温度条件下抑制的重组的反式高尔基复合体，并依赖于微管。重建的trans-Golgi复合体，破坏后的顺式/中间-高尔基复合体与ER的选择性融合诺考达唑，发生后释放顺式/中间-高尔基蛋白质从ER和顺式/中间cisterectin的组装。
We have studied the disassembly and assembly of two morphologically and functionally distinct parts of the Golgi complex, the cis/middle and trans cisterna/trans network compartments. For this purpose we have followed the redistribution of three cis/middle- (GMPc-1, GMPc-2, MG 160) and two trans- (GMPt-1 and GMPt-2) Golgi membrane proteins during and after treatment of normal rat kidney (NRK) cells with brefeldin A (BFA). BFA induced complete disassembly of the cis/middle- and trans- Golgi complex and translocation of GMPc and GMPt to the ER. Cells treated for short times (3 min) with BFA showed extensive disorganization of both cis/middle- and trans-Golgi complexes. However, complete disorganization of the trans part required much longer incubations with the drug. Upon removal of BFA the Golgi complex was reassembled by a process consisting of three steps: (a) exist of cis/middle proteins from the ER and their accumulation into vesicular structures scattered throughout the cytoplasm; (b) gradual relocation and accumulation of the trans proteins in the vesicles containing the cis/middle proteins; and (c) assembly of the cisternae, and reconstruction of the Golgi complex within an area located in the vicinity of the centrosome from which the ER was excluded. Reconstruction of the cis/middle-Golgi complex occurred under temperature conditions inhibitory of the reorganization of the trans- Golgi complex, and was dependent on microtubules. Reconstruction of the trans-Golgi complex, disrupted with nocodazole after selective fusion of the cis/middle-Golgi complex with the ER, occurred after the release of cis/middle-Golgi proteins from the ER and the assembly of the cis/middle cisternae.
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布雷菲德菌素 A 阻断小鼠垂体细胞中分泌蛋白的细胞内转运：对促甲状腺素和游离 α 亚基生物合成的影响。
影响因子：
4.8
作者：
Magner,JA;Papagiannes,E
通讯作者：
Papagiannes,E
DOI：
10.1073/pnas.86.18.6992
发表时间：
1989-09
影响因子：
11.1
作者：
Jeffrey B. Ulmer;G. Palade
通讯作者：
Jeffrey B. Ulmer;G. Palade
肠吸收细胞和杯状细胞高尔基体末端糖基化的差异亚区室。
DOI：
--
发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Roth,J;Taatjes,DJ;Weinstein,J;Paulson,JC;Greenwell,P;Watkins,WM
通讯作者：
Watkins,WM
使用针对 MG-160（大鼠高尔基体的唾液酸糖蛋白）的抗血清对多个物种（包括人类）和组织中的高尔基体进行免疫细胞化学可视化。
DOI：
10.1177/38.7.2355176
发表时间：
1990
期刊：
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
影响因子：
--
作者：
Croul,S;Mezitis,SG;Stieber,A;Chen,YJ;Gonatas,JO;Goud,B;Gonatas,NK
通讯作者：
Gonatas,NK