In vitro reconstitution of substrate S-acylation by the zDHHC family of protein acyltransferases.
In vitro reconstitution of substrate S-acylation by the zDHHC family of protein acyltransferases.
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作者：
Protein S-acylation, more commonly known as protein palmitoylation, is a biological process defined by the covalent attachment of long chain fatty acids onto cysteine residues of a protein, effectively altering the local hydrophobicity and influencing its stability, localization and overall function. Observed ubiquitously in all eukaryotes, this post translational modification is mediated by the 23-member family of zDHHC protein acyltransferases in mammals. There are thousands of proteins that are S-acylated and multiple zDHHC enzymes can potentially act on a single substrate. Since its discovery, numerous methods have been developed for the identification of zDHHC substrates and the individual members of the family that catalyse their acylation. Despite these recent advances in assay development, there is a persistent gap in knowledge relating to zDHHC substrate specificity and recognition, that can only be thoroughly addressed through in vitro reconstitution. Herein, we will review the various methods currently available for reconstitution of protein S-acylation for the purposes of identifying enzyme–substrate pairs with a particular emphasis on the advantages and disadvantages of each approach.
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影响因子：
2.9
作者：
Hamel LD;Deschenes RJ;Mitchell DA
通讯作者：
Mitchell DA
DOI：
10.1007/978-1-4939-9532-5_16
发表时间：
2019-01-01
期刊：
PROTEIN LIPIDATION: METHODS AND PROTOCOLS
影响因子：
--
作者：
Blanc, Mathieu;David, Fabrice P. A.;van der Goot, F. Gisou
通讯作者：
van der Goot, F. Gisou
影响因子：
4.8
作者：
Lobo, S;Greentree, WK;Deschenes, RJ
通讯作者：
Deschenes, RJ
影响因子：
4.1
作者：
Gadalla, Mohamed Rasheed;Abrami, Laurence;Veit, Michael
通讯作者：
Veit, Michael
影响因子：
4.1
作者：
Gonzalez Montoro, Ayelen;Quiroga, Rodrigo;Valdez Taubas, Javier
通讯作者：
Valdez Taubas, Javier