Impacts of Mesoporous Silica Nanoparticle Size, Pore Ordering, and Pore Integrity on Hemolytic Activity
Impacts of Mesoporous Silica Nanoparticle Size, Pore Ordering, and Pore Integrity on Hemolytic Activity
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DOI：
10.1021/ja910846q
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发表时间：
2010-04-07
影响因子：
15
通讯作者：
Haynes, Christy L.
中科院分区：
文献类型：
--
作者：
Lin, Yu-Shen;Haynes, Christy L.
本文采用溶血性测定方法对不同尺寸的无孔和多孔二氧化硅纳米颗粒的毒性进行了评价，并研究了多孔结构和完整性对纳米颗粒-细胞相互作用的影响。结果表明，无孔和多孔二氧化硅均以浓度和尺寸依赖性方式引起红细胞膜损伤。在介孔二氧化硅纳米颗粒的情况下，尺寸依赖性溶血效应仅存在于纳米颗粒具有长程有序的多孔结构时，这表明孔结构在细胞-纳米颗粒相互作用中是至关重要的。介孔二氧化硅纳米颗粒显示出比类似尺寸的无孔对应物更低的溶血活性，这可能是由于多孔二氧化硅纳米颗粒的细胞可接触表面上的硅烷醇基团更少。中孔二氧化硅纳米颗粒的溶血程度随着孔结构在磷酸盐缓冲溶液中的温和老化而受损而增加，从而引发中孔塌陷。通过TEM、XRD、N-2吸附-脱附等温线和溶解二氧化硅的定量来检查介孔二氧化硅纳米颗粒的孔完整性。在这些纳米颗粒中，孔稳定性显然是确定溶血活性的重要因素;进一步的研究表明，纳米颗粒诱导的溶血可以通过用聚（乙二醇）涂层修饰硅烷醇表面来消除。
This paper uses the measure of hemolysis to evaluate the toxicity of nonporous and porous silica nanoparticles with varied sizes and investigates the effects of porous structure and integrity on the nanoparticle-cell interaction. The results show that both nonporous and porous silica cause red blood cell membrane damage in a concentration- and size-dependent manner. In the case of mesoporous silica nanoparticles, the size-dependent hemolysis effect is only present when the nanoparticles have long-range ordered porous structure, revealing that pore structure is critical in cell-nanoparticle interactions. Mesoporous silica nanoparticles show lower hemolytic activity than their nonporous counterparts of similar size, likely due to fewer silanol groups on the cell-contactable surface of the porous silica nanoparticles. The extent of hemolysis by mesoporous silica nanoparticles increases as the pore structure is compromised by mild aging in phosphate-buffered solutions, initiating mesopore collapse. The pore integrity of mesoporous silica nanoparticles is examined by TEM, XRD, N-2 adsorption-desorption isotherms, and quantification of dissolved silica. In these nanoparticles, pore stability is clearly an important factor in determining the hemolytic activity; further work demonstrates that nanoparticle-induced hemolysis can be eliminated by modifying the silanol surface with a poly(ethylene glycol) coating.