Dendritic branch typing and spine expression patterns in cortical nonpyramidal cells
Dendritic branch typing and spine expression patterns in cortical nonpyramidal cells
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DOI：
10.1093/cercor/bhj015
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发表时间：
2006-05-01
期刊：
影响因子：
3.7
通讯作者：
Kubota, Y
中科院分区：
文献类型：
--
作者：
Kawaguchi, Y;Karube, F;Kubota, Y
为了了解各种类型的皮质非锥体细胞的树突分化，我们定量分析了它们的树突分支和脊柱表达。树突节间和棘间间隔服从具有特定类型衰减常数的指数分布。光镜水平上的初始分支模式、节间间隔和棘密度将非锥体细胞分为三种树突类型，与轴突、神经化学和放电类型相关。最初的分支模式决定了树突的整体垂直分布。具有不同放电和化学表达模式的篮子细胞亚型在垂直和水平空间分布上是不同的，提供了不同的输入区域。树突棘的节间密度以及轴突突触的节间密度与曲折和间隔无关，表明突触在树干上均匀分布的趋势。在电子显微镜水平上鉴定出的树突棘在不同亚型的长度和形状上有所不同。虽然密度低于锥体细胞，但刺本身也由蘑菇型、多头型等多种形态类型组成，且在亚型之间表达存在差异。树突分支特征与脊柱结构差异的相关性表明了在亚型之间接收特定输入的不同方式。
To understand the dendritic differentiation in various types of cortical nonpyramidal cells, we analyzed quantitatively their dendritic branching and spine expression. The dendritic internode and interspine interval obeyed exponential distributions with type-specific decay constants. The initial branching pattern, internode interval and spine density at the light microscopic level divided nonpyramidal cells into three dendritic types, correlated with axonal, neurochemical and firing types. The initial branching pattern determined the overall vertical spread of dendrites. Basket cell subtypes with different firing and chemical expression patterns were distinct in the vertical and horizontal spatial spread, providing diverse input territories. Internode densities of dendritic spines, as well as those of axonal synaptic boutons, did not correlate with the tortuosities and intervals, suggesting a tendency to distribute synapses homogeneously over the arbor. Dendritic spines identified at the electron microscopic level were different in length and shape among subtypes. Although the density was lower than that of pyramidal cells, spines themselves were also composed of several morphological types such as mushroom and multihead ones, which were expressed differentially among subtypes. Correlation of dendritic branching characteristics with differences in spine structure suggests distinct ways to receive specific inputs among the subtypes.