Internal RNA Replication Elements are Prevalent in Tombusviridae.
Internal RNA Replication Elements are Prevalent in Tombusviridae.
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DOI：
10.3389/fmicb.2012.00279
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发表时间：
2012
影响因子：
5.2
通讯作者：
White KA
中科院分区：
文献类型：
--
作者：
Nicholson BL;Lee PK;White KA
内部复制元件（IRE）是存在于不同类型正链RNA病毒基因组内部位置的RNA结构。Tombusvirus属（Tombusviridae科）的成员在其基因组的聚合酶编码区内含有IRE，该RNA元件参与基因组靶向复制位点和复制酶复合物组装。在这里，我们提出，其他成员的病毒家族Tombusviridae也具有可比的IRE。通过序列和结构分析，鉴定了该科几个属的候选IRE，包括金色病毒、坏死病毒、卡莫病毒和洋孢病毒。随后对选定的拟定IRE进行突变分析的结果与这些结构在感染期间病毒基因组积累中的关键作用一致。我们的研究支持存在于Tombusviridae的几个属的IREs，并指出以前不受重视的相似性，基因组复制策略之间的成员，这个病毒家族。
Internal replication elements (IREs) are RNA structures that are present at internal positions in the genomes of different types of plus-strand RNA viruses. Members of the genus Tombusvirus (family Tombusviridae) contain an IRE within the polymerase coding region of their genomes and this RNA element participates in both genome targeting to sites of replication and replicase complex assembly. Here we propose that other members of the virus family Tombusviridae also possess comparable IREs. Through sequence and structural analyses, candidate IREs in several genera of this family were identified, including aureusviruses, necroviruses, carmoviruses, and pelarspoviruses. The results from subsequent mutational analysis of selected proposed IREs were consistent with a critical role for these structures in viral genome accumulation during infections. Our study supports the existence of IREs in several genera in Tombusviridae and points to previously unappreciated similarities in genome replication strategies between members of this virus family.