GROWTH OF TRANSFORMED ROOTS IN A NUTRIENT MIST BIOREACTOR - REACTOR PERFORMANCE AND EVALUATION
GROWTH OF TRANSFORMED ROOTS IN A NUTRIENT MIST BIOREACTOR - REACTOR PERFORMANCE AND EVALUATION
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DOI：
10.1007/bf00180969
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发表时间：
1992-07-01
影响因子：
5
通讯作者：
WEATHERS, PJ
中科院分区：
文献类型：
--
作者：
DIIORIO, AA;CHEETHAM, RD;WEATHERS, PJ
对营养雾生物反应器进行了改进，用于在尼龙支架上培养甜菜根和红花根。为了在1周的时间内最大限度地提高菌种的生长，研究人员改变了喷雾周期、接种量、分批或连续操作以及蔗糖浓度等培养条件。在1.8-1培养室的营养雾中培养的根组织的生长水平与在具有相同培养基的摇瓶中培养的毛状根相当。我们的研究结果证明了营养雾培养在毛状根上的有效性，从而为这些组织的成功培养提供了另一种方法。
A nutrient mist bioreactor was modified for culturing transformed roots of Beta vulgaris and Carthamus tinctorius on a nylon support. Culture conditions of misting cycle, inoculum size, batch or continuous operation and sucrose concentration were varied in order to maximize growth over a 1-week period. Root tissue cultured in nutrient mists in a 1.8-1 culture chamber achieved levels of growth equivalent to hairy roots cultured in shake flasks with identical medium. Our results demonstrate the effectiveness of nutrient mist culture as applied to hairy roots, thereby providing an alternative means for successful culture of these tissues.