Extracellular Vesicle Levels of Nervous System Injury Biomarkers in Critically Ill Trauma Patients with and without Traumatic Brain Injury.
Extracellular Vesicle Levels of Nervous System Injury Biomarkers in Critically Ill Trauma Patients with and without Traumatic Brain Injury.
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细胞外囊泡水平的神经系统损伤生物标志物中患有和没有创伤性脑损伤的患者。
DOI：
10.1089/neur.2022.0058
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发表时间：
2022
影响因子：
2.4
通讯作者：
Hinson, Holly E.
中科院分区：
文献类型：
--
作者：
Guedes, Vivian A.;Mithani, Sara;Williams, Cydni;Sass, Dilorom;Smith, Ethan G.;Vorn, Rany;Wagner, Chelsea;Lai, Chen;Gill, Jessica;Hinson, Holly E.
中度/重度创伤性脑损伤 (TBI) 会导致具有异质病理的损伤模式，从而产生不同的恢复结果。细胞外囊泡 (EV) 是含有多种参与细胞信号转导的分子的颗粒。由于 EV 的封装性，包括提高稳定性/减少降解，EV 可能有望成为 TBI 中的生物标志物。对来自危重创伤患者的前瞻性观察试验中患有和不患有 TBI 的受试者子集进行了分析。使用单分子阵列技术测量神经胶质（胶质纤维酸性蛋白；GFAP）和神经元/轴突（泛素羧基末端水解酶 L1 [UCH-L1]、神经丝轻链 [NfL] 和总 tau）蛋白的总 EV 水平。对蛋白质水平进行缩尾处理以解决异常值并进行对数转换以进行分析。多发伤（n = 41）和孤立性身体损伤（n = 73）患者的年龄和性别相似。正如预期的那样，多处损伤的患者损伤更严重，损伤严重程度评分较高（29 [26–41] vs. 21 [14–26]，p< 0.001），格拉斯哥昏迷量表评分较低（12 [4–13] vs. 13 [13–13]，p< 0.001）。多重损伤患者的 GFAP、UCH-L1 和 NfL 全身 EV 较高（1768 [932–4780] vs. 239 [63–589]，p< 0.001；75.4 [47.8–158.3] vs. 41.5 [21.5–67.1]， p = 0.03；分别为 7.5 [3.3–12.3] 与 2.9 [2.1–4.8]，p < 0.001）。头部简短损伤评分与 GFAP 之间存在中等相关性（自由循环 rho = 0.62，EV rho = 0.64；两者 p< 0.001）。 EV 中含有的脑源性蛋白质有望成为住院患者 TBI 后生物标志物测量的一种信息方法。未来的评估和纵向研究有必要得出有关这些生物标志物的临床效用的结论。
Moderate/severe traumatic brain injury (TBI) causes injury patterns with heterogeneous pathology producing varying outcomes for recovery. Extracellular vesicles (EVs) are particles containing a myriad of molecules involved in cell signaling. EVs may hold promise as biomarkers in TBI because of their encapsulation, including improved stability/decreased degradation. A subset of subjects with and without TBI from a prospective, observational trial of critically ill trauma patients were analyzed. Total EV levels of glial (glial fibrillary acidic protein; GFAP) and neuronal/axonal (ubiquitin carboxy-terminal hydrolase L1 [UCH-L1], neurofilament light chain [NfL], and total-tau) proteins were measured using single-molecule array technology. Protein levels were winsorized to address outliers and log transformed for analysis. Patients with multiple injuries (n = 41) and isolated body injury (n = 73) were of similar age and sex. Patients with multiple injuries were, as expected, more severely injured with higher Injury Severity Scores (29 [26–41] vs. 21 [14–26], p < 0.001) and lower Glasgow Coma Scale scores (12 [4–13] vs. 13 [13–13], p < 0.001). Total body EVs of GFAP, UCH-L1, and NfL were higher in those with multiple injuries (1768 [932–4780] vs. 239 [63–589], p < 0.001; 75.4 [47.8–158.3] vs. 41.5 [21.5–67.1], p = 0.03; 7.5 [3.3–12.3] vs. 2.9 [2.1–4.8], p < 0.001, respectively). There was a moderate correlation between the Head Abbreviated Injury Score and GFAP (free circulating rho = 0.62, EV rho = 0.64; both p < 0.001). Brain-derived proteins contained in EV holds promise as an informative approach to biomarker measurement after TBI in hospitalized patients. Future evaluation and longitudinal studies are necessary to draw conclusions regarding the clinical utility of these biomarkers.
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DOI：
10.1186/cc10286
发表时间：
2011-06-24
期刊：
Critical care (London, England)
影响因子：
--
作者：
Mondello S;Papa L;Buki A;Bullock MR;Czeiter E;Tortella FC;Wang KK;Hayes RL
通讯作者：
Hayes RL
影响因子：
4.8
作者：
Beard K;Yang Z;Haber M;Flamholz M;Diaz-Arrastia R;Sandsmark D;Meaney DF;Issadore D
通讯作者：
Issadore D
影响因子：
3.4
作者：
Anderson, Taylor N.;Hwang, Jun;Rowell, Susan E.
通讯作者：
Rowell, Susan E.
影响因子：
3.4
作者：
Hinson, Holly E.;Rowell, Susan;Schreiber, Martin A.
通讯作者：
Schreiber, Martin A.
影响因子：
37.8
作者：
Mallat, Z;Hugel, B;Tedgui, A
通讯作者：
Tedgui, A