DETECTION OF IONOPHOROUS ANTIBIOTIC-CATION COMPLEXES IN WATER WITH FLUORESCENT PROBES
DETECTION OF IONOPHOROUS ANTIBIOTIC-CATION COMPLEXES IN WATER WITH FLUORESCENT PROBES
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DOI：
10.1073/pnas.68.9.2037
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发表时间：
1971-01-01
影响因子：
11.1
通讯作者：
FELSENFELD, H
中科院分区：
文献类型：
--
作者：
FEINSTEIN, MB;FELSENFELD, H
在水介质中，通过使用荧光探针1-苯胺基-8-萘磺酸盐和2-对甲苯胺基-6-萘磺酸盐，已证明离子型抗生素缬氨霉素、尼日利亚菌素、丙甲菌素和大四内酯肌动蛋白与碱金属阳离子的结合。离子载体-阳离子复合物与荧光染料的相互作用产生增强的荧光发射，增加的寿命和偏振，以及荧光光谱的发射最大值的显著蓝移。在恒定的抗生素和荧光团浓度在水中，荧光发射的强度被发现是阳离子浓度的函数。这允许测定丙甲霉素（Na+> K+）、缬氨霉素（Rb+> K+> Cs+）、尼日利亚菌素（K+> Rb+> Na+> Cs+）和trinactin（NH 4 +> K+> Rb+> Cs+）的相对阳离子亲和力。
The binding of alkali cations by the ionophorous antibiotics valinomycin, nigericin, alamethicin, and the macrotetralide actins has been shown to occur, in aqueous media, by the use of the fluorescent probes 1-anilino-8-naphthalene sulfonate and 2-p-toluidinyl-6-naphthalene sulfonate. The interaction of the ionophore-cation complexes with the fluorescent dyes produced enhanced fluorescence emission, increased lifetime and polarization, and a significant blue-shift of the emission maxima of the fluorescence spectrum. At constant antibiotic and fluorophore concentrations in water, the intensity of the fluorescence emission was found to be a function of the cation concentration. This permitted relative cation affinities to be determined for alamethicin (Na+≅ K+), valinomycin (Rb+> K+> Cs+), nigericin (K+> Rb+> Na+> Cs+) and trinactin (NH4+> K+> Rb+> Cs+).