Anti-inflammaging effects of Lactobacillus brevis OW38 in aged mice
Anti-inflammaging effects of Lactobacillus brevis OW38 in aged mice
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DOI：
10.3920/bm2016.0016
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发表时间：
2016-01-01
影响因子：
5.4
通讯作者：
Kim, D. -H.
中科院分区：
文献类型：
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作者：
Jeong, J. -J.;Kim, K. A.;Kim, D. -H.
本研究从泡菜中收集乳酸菌（LAB）菌株，筛选分离出抑制大肠杆菌产生脂多糖（LPS）和抑制LPS刺激的巨噬细胞中p16表达和核因子κ B （nf - κ B）活化的菌株。老龄小鼠（雄性，18个月大）口服短乳杆菌OW38 （1 × 10(9) cfu/只）8周，可降低结肠液和血液中的LPS水平。此外，OW38处理还降低了厚壁菌门（Firmicutes）或变形菌门（Proteobacteria）与拟杆菌门（Bacteroidetes）的比例，老年小鼠明显高于年轻小鼠。老龄小鼠用OW38处理可抑制炎症标志物的表达，如髓过氧化物酶、肿瘤坏死因子（TNF）和白细胞介素(IL)-1 β，并抑制nf - κ B的激活。此外，它还诱导结肠紧密连接蛋白zonula occludens-1、occludin和claudin-1的表达。OW38处理还抑制衰老小鼠结肠和海马中衰老标志物p16、p53和SAMHD1的表达。此外，与年轻小鼠相比，它显著恢复了老年小鼠的自发交替以及脑源性神经营养因子和双皮质素的表达(P
In the present study, lactic acid bacteria (LAB) strains were collected from kimchi and were screened to isolate strains that inhibit lipopolysaccharide (LPS) production by Escherichia coli and p16 expression and nuclear factor-kappa B (NF-kappa B) activation in LPS-stimulated macrophages. Oral administration of Lactobacillus brevis OW38 (1x10(9) cfu/mouse) to aged mice (male, 18 months old) for 8 weeks reduced the LPS level in colon fluid and blood. In addition, OW38 treatment also reduced the ratio of Firmicutes or Proteobacteria to Bacteroidetes, which was significantly higher in aged mice than in young mice. Treatment with OW38 in aged mice inhibited the expression of inflammatory markers, such as myeloperoxidase, tumour necrosis factor (TNF), and interleukin (IL)-1 beta, and inhibited NF-kappa B activation. Furthermore, it induced the expression of colonic tight junction proteins zonula occludens-1, occludin, and claudin-1. OW38 treatment also suppressed the expression of senescence markers p16, p53, and SAMHD1 in the colon and the hippocampus of aged mice. In addition, it significantly restored spontaneous alternation as well as the expression of brain-derived neurotrophic factor and doublecortin in aged mice compared to that in young mice (P