Phenotype and function of CD25-expressing B lymphocytes isolated from human umbilical cord blood.
Phenotype and function of CD25-expressing B lymphocytes isolated from human umbilical cord blood.
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DOI：
10.1155/2011/481948
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发表时间：
2011
影响因子：
--
通讯作者：
Brisslert M
中科院分区：
文献类型：
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作者：
Amu S;Brisslert M
背景。我们已经证明，大约 30% 的人类外周血 B 细胞表达 CD25。表达 CD25 的 B 细胞表现出属于记忆 B 细胞群的成熟表型，并具有更好的增殖和抗原呈递能力。本研究的目的是表征人类脐带血中表达 CD25 的 B 细胞亚群。材料和方法。来自人脐带血 (n = 34) 和成人外周血 (n = 22) 的单核细胞部分被分类为 CD20+CD25+ 和 CD20+CD25− B 细胞群。使用流式细胞术、增殖、细胞因子产生和免疫球蛋白分泌来比较这些 B 细胞群的表型和功能。结果。表达 CD25 的 B 细胞是有限的脐带血单核细胞群，占 CD20+ B 细胞的 5%。它们的特点是在脐带血中高表达CD5，在成人血液中高表达CD27。表达 CD25 的 B 细胞表达功能性 IL-2 受体和高水平的 CC 趋化因子受体，并自发产生 IgG 和 IgM 亚类抗体。结论。 CD25 表达是特定免疫调节 B 细胞亚群的共同特征，该亚群在 IL-2 刺激下准备增殖，可能准备迁移并归巢到外周组织中以进一步分化/发挥作用。
Background. We have shown that approximately 30% of human peripheral blood B-cells express CD25. B cells expressing CD25 display a mature phenotype belonging to the memory B-cell population and have a better proliferative and antigen-presenting capacity. The aim of the present study was to characterize the CD25-expressing subset of B cells in human cord blood. Material and Methods. Mononuclear cell fraction from human cord blood (n = 34) and peripheral adult blood (n = 22) was sorted into CD20+CD25+ and CD20+CD25− B-cell populations. Phenotype and function of these B-cell populations were compared using flow cytometry, proliferation, cytokine production, and immunoglobulin secretion. Results. CD25-expressing B cells are a limited population of cord blood mononuclear cells representing 5% of the CD20+ B cells. They are characterised by high expression of CD5 in cord blood and CD27 in adult blood. CD25-expressing B cells express a functional IL-2 receptor and high levels of CC-chemokine receptors and spontaneously produce antibodies of IgG and IgM subclass. Conclusions. CD25 expression is a common denominator of a specific immunomodulatory B-cell subset ready to proliferate upon IL-2 stimulation, possibly ready to migrate and home into the peripheral tissue for further differentiation/action.