The Integration Preference of Sleeping Beauty at Non-TA Site Is Related to the Transposon End Sequences.
The Integration Preference of Sleeping Beauty at Non-TA Site Is Related to the Transposon End Sequences.
复制标题
睡美人在非TA位点的整合偏好与转座子末端序列有关
DOI：
10.3389/fgene.2021.639125
复制
发表时间：
2021
影响因子：
3.7
通讯作者：
Guo Y
中科院分区：
文献类型：
--
作者：
Zhou Y;Ma G;Yang J;Gao Z;Guo Y
最近，我们证明了睡美人转座子以较低的频率整合到非TA位点。我们对SB的非TA整合进行了进一步的研究，结果表明：（1）SB可以整合到HEK293T细胞和小鼠细胞系中的非TA位点，（2）高活性转座酶SB 100X和传统的SB 11都催化非TA位点的整合，（3）SB 100X和SB 11都可以整合到HEK293T细胞和小鼠细胞系中的非TA位点。（3）非TA靶位点的共有序列仅出现在转座子末端和基因组序列之间的测序连接的相对侧，表明非TA位点的整合主要是异常整合;（4）非TA靶位点的共有序列与转座子末端序列相对应。共有序列随着转座子末端的改变而改变。这一结果表明，SB转座子末端与基因组DNA（gDNA）之间的相互作用可能参与了SB整合在非TA位点的靶位点选择。
Recently, we proved that Sleeping Beauty (SB) transposon integrates into non-TA sites at a lower frequency. Here, we performed a further study on the non-TA integration of SB and showed that (1) SB can integrate into non-TA sites in HEK293T cells as well as in mouse cell lines; (2) Both the hyperactive transposase SB100X and the traditional SB11 catalyze integrations at non-TA sites; (3) The consensus sequence of the non-TA target sites only occurs at the opposite side of the sequenced junction between the transposon end and the genomic sequences, indicating that the integrations at non-TA sites are mainly aberrant integrations; and (4) The consensus sequence of the non-TA target sites is corresponding to the transposon end sequence. The consensus sequences changed following the changes of the transposon ends. This result indicated that the interaction between the SB transposon end and genomic DNA (gDNA) may be involved in the target site selection of the SB integrations at non-TA sites.