Facile synthesis of N-acetyl-L-cysteine capped CdHgSe quantum dots and selective determination of hemoglobin.
Facile synthesis of N-acetyl-L-cysteine capped CdHgSe quantum dots and selective determination of hemoglobin.
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DOI：
10.1016/j.saa.2013.08.001
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发表时间：
2014-01
期刊：
影响因子：
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通讯作者：
Qingqing Wang;Guoqing Zhan;Chunya Li
中科院分区：
文献类型：
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作者：
Qingqing Wang;Guoqing Zhan;Chunya Li
以N-乙酰基-L-半胱氨酸（NAC）为稳定剂，通过简单的水相法合成了水分散性好、质量高、稳定的镉汞硒量子点。采用傅立叶变换红外光谱、X射线光电子能谱、能量色散X射线能谱和透射电子显微镜对所制备的NAC包覆的CdHgSe量子点进行了表征。基于NAC修饰的CdHgSe量子点的荧光猝灭，建立了一种选择性测定血红蛋白（Hb）的新方法。考察了磷酸盐缓冲溶液的pH值、量子点浓度、试剂加入顺序和反应时间等因素对NAC修饰CdHgSe量子点测定血红蛋白的影响。在最佳实验条件下，血红蛋白的浓度在4.0 × 10−9 ~ 4.4 × 10− 7 mol L− 1范围内与荧光强度的变化（ΔI）呈线性关系，检出限为2.0 × 10− 9 mol L−1。该方法已成功地用于人尿样中血红蛋白的测定。
UsingN-acetyl-l-cysteine (NAC) as a stabilizer, well water-dispersed, high-quality and stable CdHgSe quantum dots were facilely synthesized via a simple aqueous phase method. The as-prepared NAC capped CdHgSe quantum dots were thoroughly characterized by fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, energy dispersive X-ray spectroscopy and transmission electron microscopy. A novel method for the selective determination of hemoglobin (Hb) was developed based on fluorescence quenching of the NAC capped CdHgSe quantum dots. A number of key factors including pH value of phosphate buffer solution, quantum dots concentration, the adding sequence of reagents and reaction time that influence the analytical performance of the NAC capped CdHgSe quantum dots in Hb determination were investigated. Under the optimal experimental conditions, the change of fluorescence intensity (ΔI) was linearly proportional to the concentration of Hb in the range of 4.0 × 10−9–4.4 × 10−7mol L−1with a detection limit of 2.0 × 10−9mol L−1. The developed method has been successfully employed to determine Hb in human urine samples.