Characterizing the presence of neutrophil extracellular traps in neutrophilic dermatoses
Characterizing the presence of neutrophil extracellular traps in neutrophilic dermatoses
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DOI：
10.1111/exd.14360
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发表时间：
2021-05-09
影响因子：
3.6
通讯作者：
Nassar, Dany
中科院分区：
文献类型：
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作者：
Eid, Edward;Safi, Remi;Nassar, Dany
神经细胞外陷阱（NET）参与多种炎性皮肤病的发病机制。然而，在非常有限数量的患者中进行了嗜中性皮肤病的NET表征;这种局限性排除了明确的结论。在这个病例系列的57例患者中，我们比较了中性粒细胞产生NET的不同实体的嗜中性粒细胞皮肤病（17脓坏疽，37与Sweet综合征和3角膜下脓疱性皮肤病）的皮肤病变的量。使用弹性蛋白酶和瓜氨酸化组蛋白3抗体，通过福尔马林固定石蜡包埋皮肤活检组织的双重免疫荧光法鉴定NET。在所有三种疾病中，显示NETs的中性粒细胞计数均较高（PG为62.9%，SS为48.5%，角膜下脓疱性皮肤病为37.8%）。各实体之间的平均百分比差异显著，与SS相比，PG显示出显著上级NET百分比。在我们的系列中，15.8%的嗜中性皮肤病与恶性肿瘤有关，10.5%与自身免疫性疾病有关，73.7%为特发性。两种病因的NETs发生率无统计学差异。这些发现表明，NET在各种实体和不同病因的嗜中性皮肤病中大量产生。与SS相比，PG中NET的上级百分比在临床上反映在其更大的溃疡坏死性质上。
Neutrophil extracellular traps (NETs) are implicated in the pathogenesis of multiple inflammatory dermatoses. However, characterization of NETs in neutrophilic dermatoses was performed on very limited number of patients; this limitation precluded definitive conclusions. In this case series of 57 patients, we compared the amounts of neutrophils producing NETs in cutaneous lesions of different entities of neutrophilic dermatoses (17 with pyoderma gangrenosum, 37 with Sweet's syndrome and three with subcorneal pustular dermatosis). NETs were identified by double immunofluorescence on formalin-fixed paraffin-embedded skin biopsies using antibodies against elastase and citrullinated histone 3. Percentages of neutrophils showing NETs were high across all three entities (62.9% in PG, 48.5% in SS and 37.8% in subcorneal pustular dermatosis). The differences in mean percentages were significant between entities, with PG showing significantly superior percentage of NETs compared with SS. In our series, 15.8% of neutrophilic dermatoses were associated with malignancies, 10.5% with autoimmune diseases and 73.7% were idiopathic. Percentages of NETs were not statistically different between aetiologies. These findings suggest that NETs are abundantly produced in the various entities and different aetiologies of neutrophilic dermatoses. In comparison with SS, the superior percentage of NETs in PG is clinically mirrored in its greater ulceronecrotic nature.