Retrotransposon populations of Vicia species with varying genome size
Retrotransposon populations of Vicia species with varying genome size
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DOI：
10.1007/s00438-005-1141-x
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发表时间：
2005-06-01
影响因子：
3.1
通讯作者：
Flavell, AJ
中科院分区：
文献类型：
--
作者：
Hill, P;Burford, D;Flavell, AJ
已经表征了基因组大小不同的三种蚕豆属物种（蚕豆、黑穗野豌豆和蚕豆）的（非LTR）LINE和Ty 3-gypsy型LTR反转录转座子群体。在每个物种中，LINE反转录转座子包含一组复杂的、非常异质的序列，而Ty 3-gypsy元件则更加同质。通过随机基因组测序和Southern杂交分析，估计了这些物种中所有三个逆转录转座子组（Ty 1-copia，Ty 3-gypsy和LINE）的拷贝数。Ty 3-gypsy元件在所有物种中都非常多，占其基因组的18-35%。Ty 1-copia族元素的丰度稍低，LINE元素的含量更低。总的来说，这三个野豌豆属物种的20-45%的基因组由反转录转座子组成。这些数据表明，三个反转录转座子组已增殖到不同程度的成员，野豌豆属和高增殖已与反转录转座子人口的均质化。
The (non-LTR) LINE and Ty3-gypsy-type LTR retrotransposon populations of three Vicia species that differ in genome size (Vicia faba, Vicia melanops and Vicia sativa) have been characterised. In each species the LINE retrotransposons comprise a complex, very heterogeneous set of sequences, while the Ty3-gypsy elements are much more homogeneous. Copy numbers of all three retrotransposon groups (Ty1-copia, Ty3-gypsy and LINE) in these species have been estimated by random genomic sequencing and Southern hybridisation analysis. The Ty3-gypsy elements are extremely numerous in all species, accounting for 18-35% of their genomes. The Ty1-copia group elements are somewhat less abundant and LINE elements are present in still lower amounts. Collectively, 20-45% of the genomes of these three Vicia species are comprised of retrotransposons. These data show that the three retrotransposon groups have proliferated to different extents in members of the Vicia genus and high proliferation has been associated with homogenisation of the retrotransposon population.