Local IL-25 contributes to Th2-biased inflammatory profiles in nasal polyps
Local IL-25 contributes to Th2-biased inflammatory profiles in nasal polyps
复制标题
局部 IL-25 导致鼻息肉中 Th2 偏向的炎症特征。
DOI：
10.1111/all.13267
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发表时间：
2018-02-01
期刊：
影响因子：
12.4
通讯作者：
Shi, J-B.
中科院分区：
文献类型：
--
作者：
Hong, H-Y.;Chen, F-H.;Shi, J-B.
背景：IL-25被认为在慢性鼻窦炎伴鼻息肉（CRSwNP）的发病机制中起关键作用。本研究旨在评估IL-25与CRSwNP中th2偏倚炎症谱的关系。方法：收集92例CRSwNP患者鼻息肉（NP）组织和对照钩突组织，20例慢性鼻窦炎无鼻息肉（CRSsNP）患者和16例正常对照者。采用免疫组织化学、免疫荧光染色、流式细胞术、RT-qPCR和ELISA检测IL-25的表达。评估2种NP亚型（IL-25（高）和IL-25（低））的炎症特征和临床特征，并检测IL-25对体外培养的分散息肉细胞中Th2细胞因子产生的影响。结果：与对照组钩突组织相比，息肉组织中IL-25 mRNA和蛋白水平显著升高。IL-25（高）亚型表现出更高的计算机断层扫描评分、内窥镜评分和Th2反应。暴露于IL-25同时激活NP的2型先天淋巴样细胞和Th2细胞，进一步增加体外Th2细胞因子的产生。结论：局部IL-25在促进NP中th2偏倚的炎症谱中起着至关重要的作用，可能是CRSwNP患者有希望的治疗靶点。
Background: IL-25 has been proposed to play a key role in the pathogenesis of chronic rhinosinusitis with nasal polyps (CRSwNP). This study aimed to evaluate the association of IL-25 with the Th2-biased inflammatory profiles in CRSwNP.Methods: Nasal polyp (NP) tissues and control uncinate process tissues were collected from 92 patients with CRSwNP, 20 patients with chronic rhinosinusitis without nasal polyps (CRSsNP), and 16 normal control subjects. IL-25 expression was examined using immunohistochemistry and immunofluorescence staining, flow cytometry, RT-qPCR, and ELISA. The inflammatory profiles and clinical characteristics of 2 NP subtypes (IL-25(high) and IL-25(low)) were evaluated, and the effects of IL-25 on Th2 cytokine production in cultured dispersed polyp cells were examined invitro.Results: The mRNA and protein levels of IL-25 were significantly increased in the polyp tissues compared with the control uncinate process tissues. The IL-25(high) subtype showed greater computed tomography scores, endoscopic scores, and Th2 response. Exposure to IL-25 activated type 2 innate lymphoid cells and Th2 cells in NP simultaneously which further increased Th2 cytokine production invitro.Conclusions: Local IL-25 plays a crucial role in promoting Th2-biased inflammatory profiles in NP and may serve as a promising therapeutic target in CRSwNP patients.