Influence of borneol on primary mice oral fibroblasts: a penetration enhancer may be used in oral submucous fibrosis
Influence of borneol on primary mice oral fibroblasts: a penetration enhancer may be used in oral submucous fibrosis
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DOI：
10.1111/j.1600-0714.2008.00738.x
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发表时间：
2009-03-01
影响因子：
3.3
通讯作者：
Wang, Sheng
中科院分区：
文献类型：
--
作者：
Dai, Jian-Ping;Chen, Jun;Wang, Sheng
冰片具有镇痛、抗炎、抗菌、增透等作用，是一种广泛应用于食品和化妆品的添加剂。我们试图用它作为一种渗透增强剂，用于治疗口腔粘膜下纤维化（OSF）的配方。为了评估其安全性，我们研究了其对原代小鼠口腔成纤维细胞的影响。采用甲基噻唑四氮唑法、乳酸脱氢酶活性法、氯胺T法、明胶酶谱法和酶联免疫吸附法分别检测冰片对成纤维细胞增殖、细胞毒性、胶原生成、基质金属蛋白酶-2,9 （mmp -2,9）活性和金属蛋白酶1组织抑制剂（TIMP-1）生成的影响。冰片浓度在18.75 ~ 300 μ g/ml范围内显著（P < 0.05）抑制成纤维细胞生长，极显著（P < 0.01）抑制胶原生成，且呈浓度依赖性。在18.75 ~ 150 μ g/ml范围内，冰片对小鼠口腔成纤维细胞无细胞毒性作用。冰片能显著（P < 0.05）降低MMP-2活性，极显著（P < 0.01）抑制TIMP-1的产生。本研究提示冰片具有抗纤维化作用，其机制可能与其抑制成纤维细胞有丝分裂、胶原和TIMP-1的产生有关。它可以安全地用作我们治疗OSF的配方的渗透增强剂。
Borneol, a widely used food and cosmetics additive, has analgesic, anti-inflammatory, antibacterial and penetration enhancing effects. We try to use it as a penetration enhancer for a formula which we have used to treat oral submucous fibrosis (OSF). To assess its safety, we investigate its effects on primary mice oral fibroblasts.Primary mice oral fibroblasts were cultured, the effects of borneol on fibroblasts proliferation, cytotoxicity, collagen production, matrix metalloproteinases-2,9 (MMP-2,9) activities and tissue inhibitors of metalloproteinase 1 (TIMP-1) production were tested by methyl thiazolyl tetrazolium assay, lactic dehydrogenase activity assay, chloramine T method, gelatin zymography and enzyme-linked immunosorbent assay, respectively.Borneol, from 18.75 to 300 mu g/ml, could significantly (P < 0.05) decrease the growth of fibroblasts and very significantly (P < 0.01) inhibit collagen production in a concentration dependant manner. From 18.75 to 150 mu g/ml, borneol had no cytotoxicity on mice oral fibroblasts. Moreover borneol could significantly (P < 0.05) decrease MMP-2 activity, and very significantly (P < 0.01) inhibit TIMP-1 production.This study indicates that borneol has anti-fibrosis activity and the mechanism may partly be relevant to its inhibiting effects on fibroblasts mitosis, collagen and TIMP-1 production. It can be safely used as a penetration enhancer for our formula to treat OSF.