Suppressor of cytokine signalling 2 (SOCS-2) expression in breast carcinoma
Suppressor of cytokine signalling 2 (SOCS-2) expression in breast carcinoma
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DOI：
10.1136/jcp.2004.024919
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发表时间：
2005-10-01
影响因子：
3.4
通讯作者：
Taffurelli, M
中科院分区：
文献类型：
--
作者：
Farabegoli, F;Ceccarelli, C;Taffurelli, M
目的：探讨SOCS-2（细胞因子信号传导抑制因子2）蛋白在乳腺癌组织中的表达与生物病理指标和生存率的关系。方法：采用抗SOCS-2的多克隆抗体对1993 ~ 1995年50例乳腺癌标本进行分析。研究了SOCS-2蛋白的存在与乳腺癌病理中使用的临床和生物学参数的关系。采用荧光原位杂交（Fluorescence in situ hybridization， FISH）技术研究SOCS-2的表达是否与SOCS-2基因拷贝数有关。结果：SOCS-2蛋白在50例乳腺癌中有34例表达，且与低分级、低核分级和p27蛋白呈正相关。SOCS-2的表达与Ki-67、细胞周期蛋白A、视网膜母细胞瘤蛋白（pRb）和表皮生长因子受体（EGFR）呈负相关。与总生存率无关系。3份样品中检测到SOCS-2扩增。FISH信号数量与SOCS-2表达无相关性。结论：SOCS-2表达、分级、核分级、p27、Ki-67、细胞周期蛋白A、pRb和EGFR标记之间的显著相关性有力地支持了SOCS-2缺失可能与乳腺癌细胞增殖和肿瘤生长有关的假设。基因拷贝数的变化似乎不影响SOCS-2的调控和表达；可能涉及其他机制，值得进一步研究。
Aims: To investigate SOCS-2 ( suppressor of cytokine signalling 2) protein expression in breast carcinoma samples in relation to biopathological parameters and survival.Methods: A polyclonal antibody against SOCS-2 was used to study 50 archival breast carcinoma samples, collected from 1993 to 1995. The presence of SOCS-2 protein was investigated in relation to clinical and biological parameters used in breast cancer pathology. Fluorescence in situ hybridisation ( FISH) was used to study whether SOCS-2 expression was related to SOCS-2 gene copy number.Results: SOCS-2 protein was expressed in 34 of 50 breast carcinoma samples and was positively associated with low grade, low nuclear grade, and p27 protein. SOCS-2 expression was inversely related to Ki-67, cyclin A, retinoblastoma protein (pRb), and the epidermal growth factor receptor ( EGFR). No relation with overall survival was demonstrated. SOCS-2 amplification was found in three samples. No relation between the number of FISH signals and SOCS-2 expression was found.Conclusions: The significant correlation seen between SOCS-2 expression, grade, nuclear grade, p27, Ki-67, cyclin A, pRb, and EGFR labelling strongly supports the hypothesis that SOCS-2 loss might be related to cell proliferation and tumour growth in breast carcinoma. Gene copy number changes did not seem to play a role in SOCS-2 regulation and expression; other mechanisms might be involved and deserve further study.