Effect of swab composition and use of swabs versus swab-containing skim milk-tryptone-glucose-glycerol (STGG) on culture- or PCR-based detection of Streptococcus pneumoniae in simulated and clinical respiratory specimens in STGG transport medium.
Effect of swab composition and use of swabs versus swab-containing skim milk-tryptone-glucose-glycerol (STGG) on culture- or PCR-based detection of Streptococcus pneumoniae in simulated and clinical respiratory specimens in STGG transport medium.
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拭子成分和拭子使用与含脱脂乳-胰蛋白胨-葡萄糖-甘油 (STGG) 拭子对 STGG 运输介质中模拟和临床呼吸道标本中基于培养或 PCR 的肺炎链球菌检测的影响。
DOI：
10.1128/jcm.01903-07
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发表时间：
2008
影响因子：
9.4
通讯作者：
Baer,Aryeh
中科院分区：
文献类型：
--
作者：
Rubin,LorryG;Rizvi,Atqia;Baer,Aryeh
为了检测肺炎链球菌的定植，将拭子置于脱脂牛奶-色氨酸-葡萄糖-甘油（STGG）运输培养基中，然后将拭子标本或STGG培养基进行培养或进行PCR。我们评估了拭子组成的影响，并比较了用拭子和含拭子培养基检测培养物和PCR的灵敏度。将海藻酸钙、涤纶或人造丝头拭子接种肺炎球菌或浸入儿童鼻洗标本中，然后置于STGG培养基中。培养接种肺炎球菌的拭子和培养基。棉签培养的菌落明显多于培养基。人造丝拭子或培养基培养的菌落数量显著高于海藻酸钙拭子或培养基培养的菌落数量。涤纶棉签和培养基的菌落数量明显低于海藻酸钙棉签或人造丝棉签。分别从海藻酸钙拭子和培养基中提取DNA，进行PCR检测肺炎球菌。接种过肺炎的拭子或临床标本中生长的细菌。肺炎，使用拭子的敏感性至少高10倍。对于涤纶或人造丝末端拭子，使用拭子和使用培养基的PCR灵敏度之间没有一致的差异。因此，对于s的培养和pcr检测，海藻酸钙拭子可能优于STGG培养基。肺炎。对于培养物，人造丝拭子较好，涤纶拭子较差。用棉签和含棉签的培养基培养或PCR检测s。肺炎与拭子组成。
To detectStreptococcus pneumoniaecolonization, the nasopharynx is sampled using a swab placed in skim milk-tryptone-glucose-glycerol (STGG) transport medium, and then the swab specimen or STGG medium is cultured or subjected to PCR. We evaluated the effect of swab composition and compared the sensitivities of detection of culture and PCR using swabs and swab-containing medium. Calcium alginate, Dacron polyester, or rayon-tipped swabs were inoculated with pneumococci or were immersed in nasal wash specimens from children and then placed in STGG medium. Swabs and medium inoculated with pneumococci were cultured. Swabs grew significantly more colonies than medium. The number of colonies cultured from rayon swabs or medium was significantly higher than the number cultured from the calcium alginate swab or medium. The number of colonies from both the Dacron polyester swabs and medium were significantly lower than with either calcium alginate or rayon swabs. When DNA was separately extracted from the calcium alginate swab and medium and subjected to PCR for pneumococcal detection from eitherS. pneumoniae-inoculated swabs or clinical specimens that grewS. pneumoniae, the sensitivity was at least 10 times higher using the swab. With Dacron polyester or rayon-tipped swabs, there was no consistent difference between the sensitivity of PCR using swabs and that of PCR using medium. Thus, calcium alginate swabs may be superior to STGG medium for the culture and PCR-based detection ofS. pneumoniae. For culture, rayon swabs are superior and Dacron polyester swabs are inferior. The sensitivity of the swab and swab-containing medium for culture or PCR detection ofS. pneumoniaevaries with swab composition.