Fabrication of carbonate apatite honeycomb and its tissue response
Fabrication of carbonate apatite honeycomb and its tissue response
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DOI：
10.1002/jbm.a.36640
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发表时间：
2019-05-01
影响因子：
4.9
通讯作者：
Miyamoto, Youji
中科院分区：
文献类型：
--
作者：
Ishikawa, Kunio;Munar, Melvin L.;Miyamoto, Youji
碳酸磷灰石（CO（3）Ap）块可用作骨替代物，因为它可以以符合骨重建过程的方式重建为新的天然骨。在众多的多孔结构中，蜂窝状（HC）结构有利于CO（3）Ap的快速置换。本研究探讨了制备CO（3）Ap HC的可行性，并沿着观察了其在兔股骨缺损中的初始组织反应。首先，从HC模具挤出Ca（OH）（2）和蜡基粘合剂的混合物。然后，在混合的O-2-CO2气氛中，在450 ℃下加热所制造的HC以除去粘合剂和碳酸化，形成CaCO 3 HC。当CaCO 3 HC在80 ℃下浸泡在1 mol/L Na 3 PO 4溶液中7 d时，其组成从CaCO 3变为CO（3）Ap，保持原始CaCO 3 HC的结构。CaCO 3和CO（3）Ap HC的抗压强度分别为65.2 +/- 7.4 MPa和88.7 +/- 4.7 MPa。当用CO（3）Ap HC重建兔股骨骨缺损时，新骨完全穿透CO（3）Ap HC。破骨细胞和成骨细胞在新形成的骨的表面上被发现，并且骨细胞也在新形成的骨中被发现，表明正在进行的骨重建。此外，在CO（3）Ap HC的孔内形成血管。因此，CO（3）Ap HC具有良好的骨替代潜力。(c)2019 Wiley Periodicals，Inc. J Biomed Mater Res Part A：107A：1014-1020，2019.
Carbonate apatite (CO(3)Ap) block can be used as a bone substitute because it can be remodeled to new natural bone in a manner conforming with the bone remodeling process. Among the many porous structures available, honeycomb (HC) structure is advantageous for rapid replacement of CO(3)Ap to bone. In this study, the feasibility to fabricate a CO(3)Ap HC was studied, along with its initial tissue response in rabbit femur bone defect. First, a mixture of Ca(OH)(2) and a wax-based binder was extruded from a HC mold. Then the fabricated HC was heated for binder removal and carbonation at 450 degrees C in a mixed O-2-CO2 atmosphere, forming a CaCO3 HC. When the CaCO3 HC was immersed in 1 mol/L Na3PO4 solution at 80 degrees C for 7 days, its composition changed from CaCO3 to CO(3)Ap, maintaining the structure of the original CaCO3 HC. Compressive strengths of the CaCO3 and CO(3)Ap HCs were 65.2 +/- 7.4 MPa and 88.7 +/- 4.7 MPa, respectively. When the rabbit femur bone defect was reconstructed with the CO(3)Ap HC, new bone penetrated the CO(3)Ap HC completely. Osteoclasts and osteoblasts were found on the surface of the newly formed bone and osteocytes were also found in the newly formed bone, indicating ongoing bone remodeling. Furthermore, blood vessels were formed inside the pores of CO(3)Ap HC. Therefore, CO(3)Ap HC has good potential as an ideal bone substitute. (c) 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1014-1020, 2019.