fs3.1:: a major fruit shape QTL conserved in Capsicum
fs3.1:: a major fruit shape QTL conserved in Capsicum
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作者：
Ben Chaim, A;Borovsky, Y;Paran, I
Fs3.1是一个主要的果实形状(定义为果长与果宽之比)数量性状基因座(QTL)，最初是在块状和拉长果自交系之间的辣椒种内杂交中检测到的。‘毛尔’和‘常年’。除了增加果形指数外，fs3.1的‘多年生’等位基因还增加了果实伸长，降低了果实宽度和果皮厚度。我们验证了fs3.1在‘多年生’与‘毛尔’和第二个块状自交系杂交的回交自交系中的效果。为了确定fs3.1区域在辣椒额外物种中的作用，我们用‘毛尔’与结椭圆形果的辣椒BG2816杂交，以及含有从辣椒皮152225导入的fs3.1区域的导入系IL152的F-2构建了一个先进的回交群体。在两个杂交组合中都检测到了控制果实形状、果宽和果皮厚度的QTL，但没有检测到果实长度的QTL，这表明fs3.1区域作为影响辣椒果实形状的QTL是保守的。我们还测试了番茄(Lycopsicalspp.)导入系中含有相应的彭氏和毛果的fs3.1区域，但没有检测到显著的果形QTL。Fs3.1对果实尺寸生长的影响因遗传背景不同而异。通过测量不同fs3.1基因的近等基因系在果实发育过程中的子房和果实的长度和宽度，我们确定fs3.1在授粉后的前2周通过增加纵轴的生长速度来控制形状。然而，在黄花与黄花和黄花杂交中，fs3.1主要对宽度尺寸产生影响。
fs3.1 is a major fruit shape (defined as the ratio of fruit length to fruit width) quantitative trait locus (QTL) originally detected in an intraspecific cross of Capsicum annuum between the blocky and elongated-fruited inbreds. 'Maor' and 'Perennial', respectively. In addition to increasing fruit shape index, the 'Perennial' allele at fs3.1 increased fruit elongation and decreased fruit width and pericarp thickness. We verified the effect of fs3.1 in backcross inbred lines (BILs) derived from crossing 'Perennial' with 'Maor' and with a second blocky-type inbred line of C. annuum. To determine the effect of the fs3.1 region in additional Capsicum species, we constructed an advanced backcross population from the cross of 'Maor' and the oval-fruited Capsicum frutescens BG 2816 and an F-2 of the introgression line IL 152 that contains an introgression of the fs3.1 region from Capsicum chinense PI 152225. QTLs for fruit shape, fruit width, and pericarp thickness, but not for fruit length, were detected in both crosses, indicating the conservation of the fs3.1 region as a QTL affecting fruit shape in pepper. We also tested tomato (Lycopersicon spp.) introgression lines containing the corresponding fs3.1 region from L. pennellii and L. hirsutum, but we did not detect a significant fruit shape QTL in these lines. The effect of fs3.1 on the growth of fruit dimensions varied with the genetic background. By measuring the length and width of ovaries and fruits of near-isogenic C. annuum lines that differ in fS3.1 during fruit development, we determined that fs3.1 controls shape predominantly by increasing the growth rate of the longitudinal axis in the first 2 weeks after pollination. However, in the crosses of C. annuum with C. frutescens and C. chinense, fs3.1 predominantly exerted its effect on the width dimension.