Yeast surface display of lamprey variable lymphocyte receptors.
Yeast surface display of lamprey variable lymphocyte receptors.
复制标题
七鳃鳗可变淋巴细胞受体的酵母表面展示。
DOI：
10.1007/978-1-61779-139-0_2
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Pancer,Zeev
中科院分区：
文献类型：
--
作者：
Xu,Gang;Tasumi,Satoshi;Pancer,Zeev
The variable lymphocyte receptors (VLRs) of lamprey and hagfish comprise leucine-rich repeat modules, instead of the immunoglobulin-like domain building blocks of antibodies and T-cell receptors in jawed vertebrates. Both types of vertebrate-rearranging antigen receptors are similarly diverse, with repertoires that can potentially exceed 1014unique receptors. In order to characterize antigen-binding properties of the VLRs, we developed a high-throughput yeast surface display platform for the isolation of monoclonal VLRs. We have isolated VLRs that specifically bind hen egg lysozyme, β-galactosidase, cholera toxin subunit B, R-phycoerythrin, and the blood group trisaccharides A and B, with binding affinities in the mid-nanomolar to mid-picomolar range. VLRs may, thus, be excellent single-chain alternatives to Ig-based antibodies for biotechnology applications.
