The Small Heat Shock Protein HSP25/27 (HspB1) Is Abundant in Cultured Astrocytes and Associated with Astrocytic Pathology in Progressive Supranuclear Palsy and Corticobasal Degeneration.
The Small Heat Shock Protein HSP25/27 (HspB1) Is Abundant in Cultured Astrocytes and Associated with Astrocytic Pathology in Progressive Supranuclear Palsy and Corticobasal Degeneration.
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DOI：
10.1155/2010/717520
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发表时间：
2010
影响因子：
--
通讯作者：
Richter-Landsberg C
中科院分区：
文献类型：
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作者：
Schwarz L;Vollmer G;Richter-Landsberg C
神经元和神经胶质中的丝状tau阳性蛋白包涵体是许多被称为tau病的神经退行性疾病的突出特征。这些包涵体的进一步特征是存在热休克蛋白（HSPs）。这组小热休克蛋白，即HSP27和α b -晶体蛋白，与细胞骨架相互作用，与非天然蛋白结合，并在应激后阻止其聚集。为了进一步研究它们在神经退行性疾病中的作用，我们分析了HSP27与牛头病变病理病变的关系。微阵列和免疫印迹分析显示，HSP27 mRNA和蛋白水平在受影响的大脑中增强，并且与星形细胞病理有关。在伴有神经病理的牛头病中，HSP27的上调暗示了神经胶质细胞和神经元细胞的不同机制。细胞培养研究证实了这一点，表明小热休克蛋白在非应激星形胶质细胞中特异性和显著地表达，而在神经元中则不表达，并且即使在应激情况下神经元也保持相当低的水平。
Filamentous tau-positive protein inclusions in neurons and glia are prominent features of a number of neurodegenerative disorders termed tauopathies. These inclusions are further characterized by the presence of heat shock proteins (HSPs). The group of small HSPs, namely, HSP27 and αB-crystallin, interact with the cytoskeleton, bind to nonnative proteins, and prevent their aggregation after stress. To further investigate their contribution to neurodegenerative diseases, we have analyzed the association of HSP27 with pathological lesions of tauopathies. Microarray and immunoblot analysis revealed that HSP27 is enhanced at the mRNA and protein levels in affected brains, and that it is associated with astrocytic pathology. The upregulation of HSP27 in tauopathies with gial pathology implies distinct mechanisms for glial and neuronal cells. This was sustained by cell culture studies, demonstrating that the small HSPs are specifically and prominently expressed in unstressed astrocytes and not in neurons and in neurons remained at a rather low level even after stress situations.