An introduction to spectroscopy for biochemists: Edited by S B Brown. Academic press, London. 1980. £16.60 ISBN 0‐12‐137080
An introduction to spectroscopy for biochemists: Edited by S B Brown. Academic press, London. 1980. £16.60 ISBN 0‐12‐137080
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生物化学家的光谱学简介：SB Brown 学术出版社编辑，1980 年。16.60 英镑 ISBN 0‐12‐137080。
DOI：
10.1016/0307-4412(81)90077-7
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
A. Thomson
中科院分区：
文献类型：
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作者：
A. Thomson
本书是一项雄心勃勃的事业。它的目的是让非专业人士了解八种不同类型的光谱学在生物化学和临床化学领域的用途。在设计这样一本书时需要做出一些艰难的选择。首先，对于每种技术来说，重要的是要在一方面对基本原理、解释、实验特征的描述和另一方面对可以获得的对生物学家有价值的真正硬信息的有效描述之间取得正确的平衡。可能会说，潜在用户只需要被告知该技术是否可以解决他的问题。但很少有技术可以给出明确的答案。大多数涉及大量的解释以及与理论或实际模型的比较。即使是 X 射线晶体学和电子显微镜等技术，在得出结论之前也需要进行大量的解释。因此，尝试阐明一项技术（尤其是光谱技术）的内在局限性，供非光谱学家反思是很重要的。他可能会对他所读到的内容感到失望，但无论如何，他将成为一个光谱现实主义者！总的来说，这本书实现了这些理想。彼得·贝利（Peter Bayley）关于圆二色性的优雅章节树立了标准。原理和示例很好地交织在一起。这项技术在 20 世纪 60 年代被生物化学家过度吹捧，因为它是最早引入的光谱技术之一。本章纠正了平衡，并坦率地说明了什么可以得出，什么不能得出。 R Jones 的两章，一章关于核，另一章关于顺磁共振波谱学也很有用。核磁共振领域发展如此之快，以至于本文的贡献很快就会过时。 GR Penzer 对分子发射光谱进行了深入的讨论。也许令人惊讶的是，P Gans 撰写的一章振动光谱学似乎是所有讨论的技术中最具学术性且在分析意义上最无用的技术。这与该主题对低分子量材料化学的巨大影响形成鲜明对比。毫无疑问，水在红外线中的不透明度与此有关。拉曼光谱可能被低估了。编辑贡献了关于紫外和可见光谱的一章，并正确地强调了分析方面。从电子频谱中读出有用的结构信息不适合业余爱好者。规划这样一本书时要做的第二个选择是选择包含的主题。再次强调，这个选择是一个不错的选择。 M6ssbauer 和 EXAFS 等新奇产品被坚决且正确地拒绝占有一席之地。相反，其中包含了 EJ Wood 的原子吸收光谱法和 AJ Geddes 的质谱法，两者都非常值得一席之地。前一章虽然缺乏示例，但很熟练。格迪斯的最后一章对于非专业人士来说是引人入胜且令人兴奋的阅读。各种质谱技术的潜力，特别是在临床分析领域，显然是任何其他技术都无法比拟的。
This volume is an ambitious undertaking. It aims to provide, for the non-specialist, an appreciation of the uses to which eight different types of spectroscopy may be put in the fields of biochemistry and clinical chemistry. There are some difficult choices to be made in designing such a book. First, it is important to obtain for each technique the correct balance between, on the one hand, a description of underlying principles, interpretation, experimental features and, on the other, an effective discription of the really hard information of value to biologists that can be obtained. It might be said that the potential user needs only to be told whether the technique can solve his problem. But there are few techniques which give clear-cut answers. Most involve considerable interpretation and comparison with models, theoretical or actual. Even techniques such as X-ray crystallography and electron microscopy involve an enormous amount of interpretation before a conclusion can be drawn. Hence it is important to try to set out the intrinsic limitations of a technique, especially a spectroscopic one, for the non-spectroscopist to reflect upon. He may be disappointed by what he reads but nevertheless he will have become a spectroscopic realist! By and large this volume lives up to these ideals.An elegant chapter on circular dichroism by Peter Bayley sets the standard. Principles and examples are nicely interwoven. This technique was rather oversold to biochemists in the 1960s as it was one of the first spectroscopic techniques to be introduced. This chapter redresses the balance and is candid about what can be concluded and what cannot. Two chapters by R Jones, one on nuclear and the other on paramagnetic resonance spectroscopies also are useful. The field of NMR is moving so fast that this contribution will date rapidly. Molecular emission spectroscopy is discussed competently by GR Penzer. Surprisingly, perhaps, vibrational spectroscopy, a chapter by P Gans, appears as the most academic and least useful in an analytical sense of all the techniques discussed. This contrasts sharply with the enormous impact the subject had on the chemistry of low molecular weight materials. The opacity of water in the infrared, no doubt, has something to do with this. Possibly Raman spectroscopy has been undersold. The editor has contributed a chapter on ultra-violet and visible spectroscopy and rightly emphasizes the analytical aspects. The reading out of useful structural information from an electronic spectrum is not for the amateur. The second choice to be made in planning such a volume is the selection of topics for inclusion. Again the choice is a good one. The exotica such as M6ssbauer and EXAFS are firmly and rightly denied a place. Instead there is the inclusion of atomic absorption spectroscopy, by EJ Wood, and mass spectrometry, by AJ Geddes, both well worthy of a place. The former chapter is workmanlike although lacking perhaps in examples. The final chapter by Geddes makes fascinating and exciting reading for the non-specialist. The potential of the various mass spectrometric techniques, especially in the area of clinical analysis, is clearly unrivalled by any other.