Papanicolaou tests and molecular analyses using new fluid-based specimen collection technology in 3000 Japanese women
Papanicolaou tests and molecular analyses using new fluid-based specimen collection technology in 3000 Japanese women
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DOI：
10.1038/sj.bjc.6601023
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发表时间：
2003-06-16
影响因子：
8.8
通讯作者：
Nozawa, S
中科院分区：
文献类型：
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作者：
Masumoto, N;Fujii, T;Nozawa, S
为了改进样品的收集和制备，建立了一种基于液体的Papanicolaou试验。本研究是日本首次采用杂交捕获II和聚合酶链反应（PCR）技术检测基于液体的Papanicolaou标本检测人乳头瘤病毒（HPV）可行性的大规模调查。2000年10月至2001年2月期间到访庆应义塾大学医院的3000名患者参与了这项研究。液基帕帕尼科拉乌试验结果与常规帕帕尼科拉乌涂片结果吻合较好（一致性为96.8%）。液基Papanicolaou试验检测宫颈瘤变的敏感性（73.9%）和Hybrid Capture II检测宫颈瘤变的敏感性（76.3%,P = 0.55）差异无统计学意义。在宫颈上皮内瘤变3和鳞状细胞癌标本中，PCR法主要检测到HPV 16和HPV 52。虽然从液体标本中提取的一些DNA样品被降解，但即使在标本保存1年后，也可以毫不困难地进行PCR和直接测序。我们认为基于液体的Papanicolaou标本可用于HPV感染的调查。
A fluid-based Papanicolaou test has been established to improve sample collection and preparation. This study was the first large-scale investigation in Japan to examine the feasibility of using fluid-based Papanicolaou specimens to detect human papillomavirus (HPV) using Hybrid Capture II and polymerase chain reaction (PCR). Three thousand patients who visited Keio University Hospital between October 2000 and February 2001 were enrolled in the study. The results of the fluid-based Papanicolaou tests corresponded well with those of conventional Papanicolaou smears (96.8% concordance). The sensitivities of cervical neoplasia detection using the fluid-based Papanicolaou test (73.9%) and Hybrid Capture II (76.3%, P = 0.55) were not significantly different. Among the cervical intraepithelial neoplasia 3 and squamous cell carcinoma specimens, HPV 16 and HPV 52 were predominantly detected using the PCR method. Although some DNA samples extracted from the fluid-based specimens were degradaded, PCR and direct sequencing could be performed without difficulty even after 1 year of specimen storage. We conclude that fluid-based Papanicolaou specimens can be applied to investigate HPV infection.