IL-10 inhibits inflammatory cytokines released by fetal mouse lung fibroblasts exposed to mechanical stretch.
IL-10 inhibits inflammatory cytokines released by fetal mouse lung fibroblasts exposed to mechanical stretch.
复制标题
DOI：
10.1002/ppul.21433
复制
发表时间：
2011-07
影响因子：
3.1
通讯作者：
Sanchez-Esteban, Juan
中科院分区：
文献类型：
--
作者：
Hawwa, Renda L.;Hokenson, Michael A.;Wang, Yulian;Huang, Zheping;Sharma, Surendra;Sanchez-Esteban, Juan
Mechanical ventilation plays an important role in the pathogenesis of bronchopulmonary dysplasia. However, the molecular mechanisms by which excessive stretch induces lung inflammation are not well characterized. In this study, we investigated in vitro the contribution of lung mesenchymal cells to the inflammatory response mediated by mechanical stretch and the potential protective role of IL-10. Fetal mouse lung fibroblasts isolated during the saccular stage of lung development were exposed to 20% cyclic stretch to simulate mechanical injury. The phenotype of cultured fibroblasts was investigated by red oil O and alpha-smooth muscle actin staining. Cell necrosis, apoptosis and inflammation were analyzed by LDH release, cleaved caspase-3 activation and release of cytokines and chemokines into the supernatant, respectively. First, we characterized the phenotype of the cultured fibroblasts and found an absence of red oil O staining and 100% positive staining for alpha-smooth muscle actin, indicating that cultured fibroblasts were myofibroblasts. Mechanical stretch increased necrosis and apoptosis by 2- and 3-fold, compared to unstretched samples. Incubation of monolayers with IL-10 prior to stretch did not affect necrosis but significantly decreased apoptosis. Mechanical stretch increased release of pro-inflammatory cytokines and chemokines IL-1β, MCP-1, RANTES, IL-6, KC and TNF-α into the supernatant by 1.5- to 2.5-fold, and administration of IL-10 before stretch blocked that release. Our data demonstrate that lung interstitial cells may play a significant role in the inflammatory cascade triggered by mechanical stretch. IL-10 protects fetal fibroblasts from injury secondary to stretch.
登录
查看更多内容
影响因子：
2.5
作者：
Davidson, Dennis;Miskolci, Veronika;Vancurova, Ivana
通讯作者：
Vancurova, Ivana
影响因子：
3.5
作者：
Edwards, YS;Sutherland, LM;Murray, AW
通讯作者：
Murray, AW
DOI：
10.1152/ajplung.2000.279.4.l699
发表时间：
2000-10-01
影响因子：
4.9
作者：
Mourgeon, E;Isowa, N;Liu, MY
通讯作者：
Liu, MY
影响因子：
3.6
作者：
Bustani, P;Hodge, R;Kotecha, S
通讯作者：
Kotecha, S
DOI：
10.1152/ajplung.00251.2009
发表时间：
2010-01-01
影响因子：
4.9
作者：
Mokres, Lucia M.;Parai, Kakoli;Bland, Richard D.
通讯作者：
Bland, Richard D.